Differential responses of Ca-activated K channels to bradykinin in sensory neurons and F-11 cells

K. Naruse, D. S. McGehee, G. S. Oxford

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The nonapeptide bradykinin (BK) excites a subset of dorsal root ganglion (DRG) neurons with putative nociceptive functions by stimulating an inward cation current. In addition, BK stimulates various intracellular signaling pathways including an elevation of intracellular Ca2+. In a DRG neuron x neuroblastoma hybrid cell (F-11), BK stimulates similar increases in intracellular [Ca2+] and inward current but also elicits a large transient outward current through Ca2+-activated K channels. We have investigated the mechanisms underlying differential expression of outward current responses in the two cell types at the single channel level. Although K(Ca) channel activity appears in inside-out patches from both cells exposed to Ca2+, BK applied to the extrapatch membrane of cell-attached patches activates K(Ca) channels in F-11 but not DRG neurons. Whereas single K(Ca) channels are quantitatively similar in terms of conductance, voltage-dependence, and sensitivity to tetraethylammonium, they differ in sensitivity to intracellular Ca2+. Channel activation in both cells requires at least four Ca2+ ions, but half-maximal activation occurs at slightly higher [Ca2+] for DRG neurons. The shift in the Ca2+ dose-response curve combined with the steep [Ca2+] dependence of channel open probability makes it less likely that a BK-induced rise in internal [Ca2+] induced will trigger a transient outward current and resultant hyperpolarization in a DRG neuron.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume262
Issue number2 31-2
StatePublished - 1992
Externally publishedYes

Fingerprint

Spinal Ganglia
Bradykinin
Sensory Receptor Cells
Neurons
Chemical activation
Calcium-Activated Potassium Channels
Tetraethylammonium
Hybrid Cells
Neuroblastoma
Cations
Cell Membrane
Ions
Membranes
Electric potential

Keywords

  • dorsal root ganglia
  • hybridoma

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Differential responses of Ca-activated K channels to bradykinin in sensory neurons and F-11 cells. / Naruse, K.; McGehee, D. S.; Oxford, G. S.

In: American Journal of Physiology - Cell Physiology, Vol. 262, No. 2 31-2, 1992.

Research output: Contribution to journalArticle

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