Differential signaling of cyclic AMP. Opposing effects of exchange protein directly activated by cyclic AMP and cAMP-dependent protein kinase on protein kinase B activation

Fang C. Mei, Jingbo Qiao, Oxana M. Tsygankova, Judy L. Meinkoth, Lawrence Quilliam, Xiaodong Cheng

Research output: Contribution to journalArticle

225 Citations (Scopus)

Abstract

The recent discovery of Epac, a novel cAMP receptor protein, opens up a new dimension in studying cAMP-mediated cell signaling. It is conceivable that many of the cAMP functions previously attributed to cAMP-dependent protein kinase (PKA) are in fact also Epac-dependent. The finding of an additional intracellular cAMP receptor provides an opportunity to further dissect the divergent roles that cAMP exerts in different cell types. In this study, we probed cross-talk between cAMP signaling and the phosphatidylinositol 3-kinase/ PKB pathways. Specifically, we examined the modulatory effects of cAMP on PKB activity by monitoring the specific roles that Epac and PKA play individually in regulating PKB activity. Our study suggests a complex regulatory scheme in which Epac and PKA mediate the opposing effects of cAMP on PKB regulation. Activation of Epac leads to a phosphatidylinositol 3-kinase-dependent PKB activation, while stimulation of PKA inhibits PKB activity. Furthermore, activation of PKB by Epac requires the proper subcellular targeting of Epac. The opposing effects of Epac and PKA on PKB activation provide a potential mechanism for the cell type-specific differential effects of cAMP. It is proposed that the net outcome of cAMP signaling is dependent upon the dynamic abundance and distribution of intracellular Epac and PKA.

Original languageEnglish (US)
Pages (from-to)11497-11504
Number of pages8
JournalJournal of Biological Chemistry
Volume277
Issue number13
DOIs
StatePublished - Mar 29 2002
Externally publishedYes

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Proto-Oncogene Proteins c-akt
Cyclic AMP-Dependent Protein Kinases
Cyclic AMP
Protein Kinases
Chemical activation
Phosphatidylinositol 3-Kinase
Proteins
Cyclic AMP Receptors
Cyclic AMP Receptor Protein
Cell signaling
Thermodynamic properties
Monitoring

ASJC Scopus subject areas

  • Biochemistry

Cite this

Differential signaling of cyclic AMP. Opposing effects of exchange protein directly activated by cyclic AMP and cAMP-dependent protein kinase on protein kinase B activation. / Mei, Fang C.; Qiao, Jingbo; Tsygankova, Oxana M.; Meinkoth, Judy L.; Quilliam, Lawrence; Cheng, Xiaodong.

In: Journal of Biological Chemistry, Vol. 277, No. 13, 29.03.2002, p. 11497-11504.

Research output: Contribution to journalArticle

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