Differential staining of DNA and RNA.

Zbigniew Darzynkiewicz, Gloria Juan, Edward F. Srour

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cell cycle analysis by means of differential staining of RNA and DNA permits determination of RNA content, which in turn allows one to discriminate G(0) versus G(1) cells and to detect cell differentiation. This unit presents two protocols for differential staining, one using the metachromatic dye acridine orange (AO) and the other a combination of pyronin Y (PY) and Hoechst 33342. Each method has its advantages and limitations, and the Hoechst-PY method is not applicable to single-laser instruments. A third protocol describes staining of viable cells to identify/sort hematopoietic stem cells, with an alternative that includes simultaneous immunostaining.

Original languageEnglish (US)
Pages (from-to)Unit 7.3
JournalCurrent protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]
VolumeChapter 7
StatePublished - Nov 2004

Fingerprint

Pyronine
RNA
Staining and Labeling
Acridine Orange
DNA
Stem cells
Gastrin-Secreting Cells
Coloring Agents
Cells
Hematopoietic Stem Cells
Lasers
Cell Differentiation
Cell Cycle

ASJC Scopus subject areas

  • Biochemistry
  • Histology
  • Medical Laboratory Technology

Cite this

Differential staining of DNA and RNA. / Darzynkiewicz, Zbigniew; Juan, Gloria; Srour, Edward F.

In: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.], Vol. Chapter 7, 11.2004, p. Unit 7.3.

Research output: Contribution to journalArticle

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