Differentiation autoantigen of testicular cells and spermatozoa in the guinea pig

Kenneth S K Tung, Liang Po Bebe Han, Andrew Evan

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Guinea pigs, immunized with autologous testes, produce antibody that reacts with surface antigen(s) of epididymal sperm and testicular cells. Both IgG and F(ab')2 fragments of the antibody molecules bind to testicular cells. Only 50% of the testicular cells had detectable surface antigens and they are identified by rosette formation with Staphylococcus aureus, which bind only to testicular cells and sperm coated with guinea pig IgG. Rosette-positive testicular cells (percentage of cells positive) are: early round spermatids (8%), oval late spermatids (98%), cytoplasmic droplets (93%), and heads (and midpieces) of testicular and epididymal sperm (100%). As determined by a quantitative cellular radioimmunobinding assay, antibody to testicular cells is absorbed by testis or sperm but not by other male or female guinea pig tissues. The antigen, which could be multiple, is therefore sperm and late spermatid specific, and is designated testicular cell-sperm differentiation autoantigen. The surface distribution of this antigen is modulated by antibody to exhibit temperature-dependent translational movement along the plasma membrane.

Original languageEnglish (US)
Pages (from-to)224-238
Number of pages15
JournalDevelopmental Biology
Volume68
Issue number1
DOIs
StatePublished - 1979
Externally publishedYes

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Autoantigens
Spermatozoa
Guinea Pigs
Spermatids
Surface Antigens
Testis
Antibodies
Immunoglobulin G
Rosette Formation
Immunoglobulin Fragments
Staphylococcus aureus
Cell Differentiation
Head
Cell Membrane
Antigens
Temperature

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Differentiation autoantigen of testicular cells and spermatozoa in the guinea pig. / Tung, Kenneth S K; Han, Liang Po Bebe; Evan, Andrew.

In: Developmental Biology, Vol. 68, No. 1, 1979, p. 224-238.

Research output: Contribution to journalArticle

Tung, Kenneth S K ; Han, Liang Po Bebe ; Evan, Andrew. / Differentiation autoantigen of testicular cells and spermatozoa in the guinea pig. In: Developmental Biology. 1979 ; Vol. 68, No. 1. pp. 224-238.
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