Differentiation of pluripotent stem cells into endothelial cells

Research output: Contribution to journalReview article

31 Scopus citations

Abstract

Purpose of review Methods to isolate endothelial cells from murine and human pluripotent stem cells continue to evolve and increasingly diverse endothelial cell populations have been generated. This review provides an update of key articles published within the past year that report on some of those advances. Recent findings Cooperative interactions among microRNA (miRNA), transcription factors and some downstream interacting proteins have been reported to enhance endothelial specification from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Endothelial cell differentiation can also be modulated by various growth factor additions, Notch pathway activation or inhibition, and modulation of the microenvironment of the differentiating ESC and iPSC. Functionality of the derived endothelium has been demonstrated by a variety of in-vitro and in-vivo assays. Finally, two recent reports have identified endothelial progenitor populations with robust proliferative potential. Summary Progress in differentiating endothelial cells from ESC and iPSC has been made. The recent report of formation of endothelial colony forming cells from human ESC and iPSC provides a protocol that can generate clinically relevant numbers of cells for human cell therapy.

Original languageEnglish (US)
Pages (from-to)252-257
Number of pages6
JournalCurrent opinion in hematology
Volume22
Issue number3
DOIs
StatePublished - May 27 2015

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Keywords

  • embryonic stem cells
  • endothelial cells
  • endothelial colony forming cells
  • induced pluripotent stem cells

ASJC Scopus subject areas

  • Hematology

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