The infection-structure (INF) genes fromUromyces appendiculatus (bean rust fungus) are related to formation of the infection structures. To determine if these genes have homologs in other fungi, genomic DNAs from selected Ascomycetes and Basidiomycetes were probed with fragments ofINF24 andINF56. At high stringency,INF24 hybridized strongly with DNA fromU. appendiculatus, but only weakly with DNA fromP. graminis tritici; and hybridization was not detected with DNA fromU. vignae (cowpea rust fungus). In contrast,INF56 hybridized with DNA fromU. appendiculatus andU. vignae but not with DNA fromP. graminis tritici. UAM, a constitutively expressed gene fromP. graminis tritici, hybridized with DNA from all three species of rust fungi. At high stringency, none of the probes hybridized with DNA from nonrust fungi, but at low stringency,INF56 hybridized weakly with DNA from most of them. In general, the results suggest that the rust genes are not broadly conserved among the fungi.
- infection structures, rust fungi
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology