Dimensionally stable and bioactive membrane for guided bone regeneration: An in vitro study

Matthew J. Rowe, Krzysztof Kamocki, Divya Pankajakshan, Ding Li, Angela Bruzzaniti, Vinoy Thomas, Steven Blanchard, Marco C. Bottino

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Composite fibrous electrospun membranes based on poly(dl-lactide) (PLA) and poly(ε-caprolactone) (PCL) were engineered to include borate bioactive glass (BBG) for the potential purposes of guided bone regeneration (GBR). The fibers were characterized using scanning and transmission electron microscopies, which respectively confirmed the submicron fibrous arrangement of the membranes and the successful incorporation of BBG particles. Selected mechanical properties of the membranes were evaluated using the suture pullout test. The addition of BBG at 10 wt % led to similar stiffness, but more importantly, it led to a significantly stronger (2.37±0.51 N mm) membrane when compared with the commercially available Epiguide® (1.06±0.24 N mm) under hydrated conditions. Stability (shrinkage) was determined after incubation in a phosphate buffer solution from 24 h up to 9 days. The dimensional stability of the PLA:PCL-based membranes with or without BBG incorporation (10.07-16.08%) was similar to that of Epiguide (14.28%). Cell proliferation assays demonstrated a higher rate of preosteoblasts proliferation on BBG-containing membranes (6.4-fold) over BBG-free membranes (4- to 5.8-fold) and EpiGuide (4.5-fold), following 7 days of in vitro culture. Collectively, our results demonstrated the ability to synthesize, via electrospinning, stable, polymer-based submicron fibrous BBG-containing membranes capable of sustaining osteoblastic attachment and proliferation-a promising attribute in GBR.

Original languageEnglish (US)
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
DOIs
StateAccepted/In press - 2015

Fingerprint

Bioactive glass
Borates
Bone
Membranes
Fibrous membranes
Composite membranes
Dimensional stability
Cell proliferation
Electrospinning
Assays
Buffers
Polymers
Phosphates
Stiffness
Transmission electron microscopy
Mechanical properties
Scanning electron microscopy
Fibers

Keywords

  • Bioglass
  • Bone
  • Electrospinning
  • Membranes
  • Regeneration

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Dimensionally stable and bioactive membrane for guided bone regeneration : An in vitro study. / Rowe, Matthew J.; Kamocki, Krzysztof; Pankajakshan, Divya; Li, Ding; Bruzzaniti, Angela; Thomas, Vinoy; Blanchard, Steven; Bottino, Marco C.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, 2015.

Research output: Contribution to journalArticle

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