Direct activation of trpl cation channels by Gα11 subunits

Alexander Obukhov, Christian Harteneck, Andrea Zobel, Rainer Harhammer, Frank Kalkbrenner, Daniela Leopoldt, Andreas Lückhoff, Bernd Nürnberg, Günter Schultz

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

G proteins of the G(q/11) subfamily functionally couple cell surface receptors to phospholipase C β (PLC β) isoforms. Stimulation of PLC β induces Ca2+ elevation by inositol 1,4,5-trisphosphate (InsP3)-mediated Ca2+ release and store-dependent 'capacitative' Ca2+ entry through Ca2+-permeable channels. The Drosophila trp gene, as well as some human trp homologs, code for such store-operated channels. The related trp-like (trpl) gene product also forms a Ca2+-permeable cation channel, but is not activated by store depletion. Co-expression of the constitutively active G(q) subfamily member Gα11 (Gα(*)11) with trpl enhanced trpl currents 33-fold in comparison with co-expression of trpl with other Gα isoforms or Gβγ complexes. This activation could not be attributed to signals downstream of PLC β. In particular, InsP3 infusion, modulation of protein kinase C activity or elevation of intracellular calcium concentration failed to induce trpl currents. In contrast, purified Gα(*)11 (but not other G protein subunits) activated trpl channels in inside-out patches. We conclude that trpl is regulated by G11 proteins in a membrane-confined manner not involving cytosolic factors. Thus, G proteins of the G(q) subfamily may induce Ca2+ entry not only indirectly via store-operated mechanisms but also by directly stimulating cation channels.

Original languageEnglish (US)
Pages (from-to)5833-5838
Number of pages6
JournalEMBO Journal
Volume15
Issue number21
StatePublished - Nov 1 1996
Externally publishedYes

Fingerprint

Type C Phospholipases
GTP-Binding Proteins
Cations
Chemical activation
Protein Isoforms
Genes
Inositol 1,4,5-Trisphosphate
Protein Subunits
Cell Surface Receptors
Protein Kinase C
Drosophila
Modulation
Calcium
Membranes
Proteins

Keywords

  • G proteins
  • Ion channels
  • Signal transduction

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Obukhov, A., Harteneck, C., Zobel, A., Harhammer, R., Kalkbrenner, F., Leopoldt, D., ... Schultz, G. (1996). Direct activation of trpl cation channels by Gα11 subunits. EMBO Journal, 15(21), 5833-5838.

Direct activation of trpl cation channels by Gα11 subunits. / Obukhov, Alexander; Harteneck, Christian; Zobel, Andrea; Harhammer, Rainer; Kalkbrenner, Frank; Leopoldt, Daniela; Lückhoff, Andreas; Nürnberg, Bernd; Schultz, Günter.

In: EMBO Journal, Vol. 15, No. 21, 01.11.1996, p. 5833-5838.

Research output: Contribution to journalArticle

Obukhov, A, Harteneck, C, Zobel, A, Harhammer, R, Kalkbrenner, F, Leopoldt, D, Lückhoff, A, Nürnberg, B & Schultz, G 1996, 'Direct activation of trpl cation channels by Gα11 subunits', EMBO Journal, vol. 15, no. 21, pp. 5833-5838.
Obukhov A, Harteneck C, Zobel A, Harhammer R, Kalkbrenner F, Leopoldt D et al. Direct activation of trpl cation channels by Gα11 subunits. EMBO Journal. 1996 Nov 1;15(21):5833-5838.
Obukhov, Alexander ; Harteneck, Christian ; Zobel, Andrea ; Harhammer, Rainer ; Kalkbrenner, Frank ; Leopoldt, Daniela ; Lückhoff, Andreas ; Nürnberg, Bernd ; Schultz, Günter. / Direct activation of trpl cation channels by Gα11 subunits. In: EMBO Journal. 1996 ; Vol. 15, No. 21. pp. 5833-5838.
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