Direct effects of HIV-1 tat on excitability and survival of primary dorsal root ganglion neurons

Possible contribution to HIV-1-associated pain

Xianxun Chi, Tohti Amet, Daniel Byrd, Kuei Hua Chang, Kavita Shah, Ningjie Hu, Ayslinn Grantham, Sishun Hu, Jianhong Duan, Feng Tao, Grant Nicol, Andy Yu

Research output: Contribution to journalArticle

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Abstract

The vast majority of people living with human immunodeficiency virus type 1 (HIV-1) have pain syndrome, which has a significant impact on their quality of life. The underlying causes of HIV-1-associated pain are not likely attributable to direct viral infection of the nervous system due to the lack of evidence of neuronal infection by HIV-1. However, HIV-1 proteins are possibly involved as they have been implicated in neuronal damage and death. The current study assesses the direct effects of HIV-1 Tat, one of potent neurotoxic viral proteins released from HIV-1-infected cells, on the excitability and survival of rat primary dorsal root ganglion (DRG) neurons. We demonstrated that HIV-1 Tat triggered rapid and sustained enhancement of the excitability of small-diameter rat primary DRG neurons, which was accompanied by marked reductions in the rheobase and resting membrane potential (RMP), and an increase in the resistance at threshold (R Th). Such Tat-induced DRG hyperexcitability may be a consequence of the inhibition of cyclin-dependent kinase 5 (Cdk5) activity. Tat rapidly inhibited Cdk5 kinase activity and mRNA production, and roscovitine, a well-known Cdk5 inhibitor, induced a very similar pattern of DRG hyperexcitability. Indeed, pre-application of Tat prevented roscovitine from having additional effects on the RMP and action potentials (APs) of DRGs. However, Tat-mediated actions on the rheobase and R Th were accelerated by roscovitine. These results suggest that Tat-mediated changes in DRG excitability are partly facilitated by Cdk5 inhibition. In addition, Cdk5 is most abundant in DRG neurons and participates in the regulation of pain signaling. We also demonstrated that HIV-1 Tat markedly induced apoptosis of primary DRG neurons after exposure for longer than 48 h. Together, this work indicates that HIV-1 proteins are capable of producing pain signaling through direct actions on excitability and survival of sensory neurons.

Original languageEnglish
Article numbere24412
JournalPLoS One
Volume6
Issue number9
DOIs
StatePublished - Sep 2 2011

Fingerprint

Spinal Ganglia
Human immunodeficiency virus 1
Viruses
Neurons
HIV-1
pain
Cyclin-Dependent Kinase 5
neurons
cyclin-dependent kinase
Pain
Human Immunodeficiency Virus Proteins
membrane potential
Membrane Potentials
Rats
Membranes
sensory neurons
Diagnosis-Related Groups
viral proteins
rats
Neurology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Direct effects of HIV-1 tat on excitability and survival of primary dorsal root ganglion neurons : Possible contribution to HIV-1-associated pain. / Chi, Xianxun; Amet, Tohti; Byrd, Daniel; Chang, Kuei Hua; Shah, Kavita; Hu, Ningjie; Grantham, Ayslinn; Hu, Sishun; Duan, Jianhong; Tao, Feng; Nicol, Grant; Yu, Andy.

In: PLoS One, Vol. 6, No. 9, e24412, 02.09.2011.

Research output: Contribution to journalArticle

Chi, Xianxun ; Amet, Tohti ; Byrd, Daniel ; Chang, Kuei Hua ; Shah, Kavita ; Hu, Ningjie ; Grantham, Ayslinn ; Hu, Sishun ; Duan, Jianhong ; Tao, Feng ; Nicol, Grant ; Yu, Andy. / Direct effects of HIV-1 tat on excitability and survival of primary dorsal root ganglion neurons : Possible contribution to HIV-1-associated pain. In: PLoS One. 2011 ; Vol. 6, No. 9.
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