Direct effects of transforming growth factor-β on chondrocytes are modulated by vitamin D metabolites in a cell maturation-specific manner

Z. Schwartz, Lynda Bonewald, K. Caulfield, B. Brooks, B. D. Boyan

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Chondrocytes in the endochondral differentiation pathway produce transforming growth factor-β (TGF-β) and respond to this growth factor both in vitro and in vivo. To clarify the role that cell maturation state plays in the response, we used a well characterized chondrocyte cell culture model which compares cartilage cells at two different stages of maturation. Confluent fourth passage cultures of rat costochondral resting zone and growth zone cartilage cells were incubated with recombinant human (rh) TGF- β-1 for 24, 48, or 72 h, and the effect on cell number and [3H]thymidine incorporation was observed. To assess whether TGF-β regulates chondrocyte differentiation to a calcifying cartilage phenotype, cells were incubated for 24 h with TGF-β, and the specific activities of alkaline phosphatase and phospholipase A2, two enzymes associated with calcification, were assayed in isolated plasma membranes and matrix vesicles. Alkaline phosphatase-specific activity was also measured in the cell layer. Modulation of TGF-β action by vitamin D metabolites, also known to regulate endochondral differentiation, was examined. The ability of the chondrocytes to produce latent TGF-β was assayed. The results show that: 1) quiescent chondrocytes at two stages of endochondral maturation respond to rhTGF-β-1 by increasing [3H]thymidine incorporation; 2) growth zone cells exhibit no increase in cell number over a 72-h incubation with TGF-β, whereas resting zone cells exhibit a dose- dependent increase in cell number at 72 h; 3) nonquiescent cells exhibit an increase in alkaline phosphatase-specific activity at 24 h; 4) the effects on this membrane enzyme are comparable in the plasma membranes and matrix vesicles, but the net effect is greater in the extracellular organelle due to the intrinsically higher levels of activity; 5) although differentiation is promoted in resting zone cells, it is limited in the growth zone cells by inhibition of phospholipase A2 activity; and 6) there is a synergistic enhancement of resting zone chondrocyte differentiation when cells are exposed to rhTGF-β-1 and 24,25-dihydroxyvitamin D3.

Original languageEnglish (US)
Pages (from-to)1544-1552
Number of pages9
JournalEndocrinology
Volume132
Issue number4
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Transforming Growth Factors
Chondrocytes
Vitamin D
Cartilage
Alkaline Phosphatase
Cell Count
Phospholipases A2
Thymidine
Growth
Cell Membrane
Calcitriol
Enzymes
Organelles
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Cell Culture Techniques
Phenotype
Membranes

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Direct effects of transforming growth factor-β on chondrocytes are modulated by vitamin D metabolites in a cell maturation-specific manner. / Schwartz, Z.; Bonewald, Lynda; Caulfield, K.; Brooks, B.; Boyan, B. D.

In: Endocrinology, Vol. 132, No. 4, 1993, p. 1544-1552.

Research output: Contribution to journalArticle

@article{3e8db13eb5514624a8d3b8a635fd1cbf,
title = "Direct effects of transforming growth factor-β on chondrocytes are modulated by vitamin D metabolites in a cell maturation-specific manner",
abstract = "Chondrocytes in the endochondral differentiation pathway produce transforming growth factor-β (TGF-β) and respond to this growth factor both in vitro and in vivo. To clarify the role that cell maturation state plays in the response, we used a well characterized chondrocyte cell culture model which compares cartilage cells at two different stages of maturation. Confluent fourth passage cultures of rat costochondral resting zone and growth zone cartilage cells were incubated with recombinant human (rh) TGF- β-1 for 24, 48, or 72 h, and the effect on cell number and [3H]thymidine incorporation was observed. To assess whether TGF-β regulates chondrocyte differentiation to a calcifying cartilage phenotype, cells were incubated for 24 h with TGF-β, and the specific activities of alkaline phosphatase and phospholipase A2, two enzymes associated with calcification, were assayed in isolated plasma membranes and matrix vesicles. Alkaline phosphatase-specific activity was also measured in the cell layer. Modulation of TGF-β action by vitamin D metabolites, also known to regulate endochondral differentiation, was examined. The ability of the chondrocytes to produce latent TGF-β was assayed. The results show that: 1) quiescent chondrocytes at two stages of endochondral maturation respond to rhTGF-β-1 by increasing [3H]thymidine incorporation; 2) growth zone cells exhibit no increase in cell number over a 72-h incubation with TGF-β, whereas resting zone cells exhibit a dose- dependent increase in cell number at 72 h; 3) nonquiescent cells exhibit an increase in alkaline phosphatase-specific activity at 24 h; 4) the effects on this membrane enzyme are comparable in the plasma membranes and matrix vesicles, but the net effect is greater in the extracellular organelle due to the intrinsically higher levels of activity; 5) although differentiation is promoted in resting zone cells, it is limited in the growth zone cells by inhibition of phospholipase A2 activity; and 6) there is a synergistic enhancement of resting zone chondrocyte differentiation when cells are exposed to rhTGF-β-1 and 24,25-dihydroxyvitamin D3.",
author = "Z. Schwartz and Lynda Bonewald and K. Caulfield and B. Brooks and Boyan, {B. D.}",
year = "1993",
doi = "10.1210/en.132.4.1544",
language = "English (US)",
volume = "132",
pages = "1544--1552",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "4",

}

TY - JOUR

T1 - Direct effects of transforming growth factor-β on chondrocytes are modulated by vitamin D metabolites in a cell maturation-specific manner

AU - Schwartz, Z.

AU - Bonewald, Lynda

AU - Caulfield, K.

AU - Brooks, B.

AU - Boyan, B. D.

PY - 1993

Y1 - 1993

N2 - Chondrocytes in the endochondral differentiation pathway produce transforming growth factor-β (TGF-β) and respond to this growth factor both in vitro and in vivo. To clarify the role that cell maturation state plays in the response, we used a well characterized chondrocyte cell culture model which compares cartilage cells at two different stages of maturation. Confluent fourth passage cultures of rat costochondral resting zone and growth zone cartilage cells were incubated with recombinant human (rh) TGF- β-1 for 24, 48, or 72 h, and the effect on cell number and [3H]thymidine incorporation was observed. To assess whether TGF-β regulates chondrocyte differentiation to a calcifying cartilage phenotype, cells were incubated for 24 h with TGF-β, and the specific activities of alkaline phosphatase and phospholipase A2, two enzymes associated with calcification, were assayed in isolated plasma membranes and matrix vesicles. Alkaline phosphatase-specific activity was also measured in the cell layer. Modulation of TGF-β action by vitamin D metabolites, also known to regulate endochondral differentiation, was examined. The ability of the chondrocytes to produce latent TGF-β was assayed. The results show that: 1) quiescent chondrocytes at two stages of endochondral maturation respond to rhTGF-β-1 by increasing [3H]thymidine incorporation; 2) growth zone cells exhibit no increase in cell number over a 72-h incubation with TGF-β, whereas resting zone cells exhibit a dose- dependent increase in cell number at 72 h; 3) nonquiescent cells exhibit an increase in alkaline phosphatase-specific activity at 24 h; 4) the effects on this membrane enzyme are comparable in the plasma membranes and matrix vesicles, but the net effect is greater in the extracellular organelle due to the intrinsically higher levels of activity; 5) although differentiation is promoted in resting zone cells, it is limited in the growth zone cells by inhibition of phospholipase A2 activity; and 6) there is a synergistic enhancement of resting zone chondrocyte differentiation when cells are exposed to rhTGF-β-1 and 24,25-dihydroxyvitamin D3.

AB - Chondrocytes in the endochondral differentiation pathway produce transforming growth factor-β (TGF-β) and respond to this growth factor both in vitro and in vivo. To clarify the role that cell maturation state plays in the response, we used a well characterized chondrocyte cell culture model which compares cartilage cells at two different stages of maturation. Confluent fourth passage cultures of rat costochondral resting zone and growth zone cartilage cells were incubated with recombinant human (rh) TGF- β-1 for 24, 48, or 72 h, and the effect on cell number and [3H]thymidine incorporation was observed. To assess whether TGF-β regulates chondrocyte differentiation to a calcifying cartilage phenotype, cells were incubated for 24 h with TGF-β, and the specific activities of alkaline phosphatase and phospholipase A2, two enzymes associated with calcification, were assayed in isolated plasma membranes and matrix vesicles. Alkaline phosphatase-specific activity was also measured in the cell layer. Modulation of TGF-β action by vitamin D metabolites, also known to regulate endochondral differentiation, was examined. The ability of the chondrocytes to produce latent TGF-β was assayed. The results show that: 1) quiescent chondrocytes at two stages of endochondral maturation respond to rhTGF-β-1 by increasing [3H]thymidine incorporation; 2) growth zone cells exhibit no increase in cell number over a 72-h incubation with TGF-β, whereas resting zone cells exhibit a dose- dependent increase in cell number at 72 h; 3) nonquiescent cells exhibit an increase in alkaline phosphatase-specific activity at 24 h; 4) the effects on this membrane enzyme are comparable in the plasma membranes and matrix vesicles, but the net effect is greater in the extracellular organelle due to the intrinsically higher levels of activity; 5) although differentiation is promoted in resting zone cells, it is limited in the growth zone cells by inhibition of phospholipase A2 activity; and 6) there is a synergistic enhancement of resting zone chondrocyte differentiation when cells are exposed to rhTGF-β-1 and 24,25-dihydroxyvitamin D3.

UR - http://www.scopus.com/inward/record.url?scp=0027538053&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027538053&partnerID=8YFLogxK

U2 - 10.1210/en.132.4.1544

DO - 10.1210/en.132.4.1544

M3 - Article

C2 - 8462452

AN - SCOPUS:0027538053

VL - 132

SP - 1544

EP - 1552

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 4

ER -