Direct pulsed field gel electrophoresis of Wilms' tumors shows that DNA deletions in 11p13 are rare

B. Royer-Pokora, Susanne Ragg, B. Heckl-Ostreicher, M. Held, U. Loos, K. Call, T. Glaser, D. Housman, G. Saunders, B. Zabel, B. Williams, A. Poustka

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Abstract

In order to search for small tumor-specific deletions in 11p13 we analysed DNA isolated from 30 fresh Wilms' tumor (WT) samples with pulsed field gel electrophoresis. For these studies we have isolated new probes from the ends of several Not1 fragments. Using these and previously described probes from 11p13 we first completed and extended the existing map of the 11p13 region. The analysis of the tumor material showed that (1) tumor-specific deletions were very rare: one homozygous deletion out of 30 tumors analysed, (2) hemizygous deletions were not observed in any of the tumors. The homozygous deletion in one patient spans 220 kb and is composed of a tumor-specific translocation associated with a deletion on one chromosome and a deletion of about 220 kb on the other chromosome at the same site. The WT-33 Wilms' tumor candidate gene maps to this deleted segment. A small constitutional deletion of 1,300 kb was identified in a patient with WT and genital tract malformations. These results suggest that in the majority of sporadic WT loss of gene function is due to subtle alterations in the gene, e.g., point mutations or very small deletions.

Original languageEnglish (US)
Pages (from-to)89-100
Number of pages12
JournalGenes Chromosomes and Cancer
Volume3
Issue number2
StatePublished - 1991
Externally publishedYes

Fingerprint

Wilms Tumor
Pulsed Field Gel Electrophoresis
DNA
Wilms' Tumor Genes
Neoplasms
Chromosome Deletion
Point Mutation
Partial deletion Chromosome 11p
Chromosomes
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Royer-Pokora, B., Ragg, S., Heckl-Ostreicher, B., Held, M., Loos, U., Call, K., ... Poustka, A. (1991). Direct pulsed field gel electrophoresis of Wilms' tumors shows that DNA deletions in 11p13 are rare. Genes Chromosomes and Cancer, 3(2), 89-100.

Direct pulsed field gel electrophoresis of Wilms' tumors shows that DNA deletions in 11p13 are rare. / Royer-Pokora, B.; Ragg, Susanne; Heckl-Ostreicher, B.; Held, M.; Loos, U.; Call, K.; Glaser, T.; Housman, D.; Saunders, G.; Zabel, B.; Williams, B.; Poustka, A.

In: Genes Chromosomes and Cancer, Vol. 3, No. 2, 1991, p. 89-100.

Research output: Contribution to journalArticle

Royer-Pokora, B, Ragg, S, Heckl-Ostreicher, B, Held, M, Loos, U, Call, K, Glaser, T, Housman, D, Saunders, G, Zabel, B, Williams, B & Poustka, A 1991, 'Direct pulsed field gel electrophoresis of Wilms' tumors shows that DNA deletions in 11p13 are rare', Genes Chromosomes and Cancer, vol. 3, no. 2, pp. 89-100.
Royer-Pokora, B. ; Ragg, Susanne ; Heckl-Ostreicher, B. ; Held, M. ; Loos, U. ; Call, K. ; Glaser, T. ; Housman, D. ; Saunders, G. ; Zabel, B. ; Williams, B. ; Poustka, A. / Direct pulsed field gel electrophoresis of Wilms' tumors shows that DNA deletions in 11p13 are rare. In: Genes Chromosomes and Cancer. 1991 ; Vol. 3, No. 2. pp. 89-100.
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AU - Glaser, T.

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