Direct visualization of transplanted hematopoietic cellreconstitution in intact mouse organs indicates the presence of a niche

Momoko Yoshimoto, Takashi Shinohara, Toshio Heike, Mitsutaka Shiota, Mito Kanatsu-Shinohara, Tatsutoshi Nakahata

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Objective. The temporal and spatial behavior of transplanted hematopoietic stem cells (HSCs) within bones remains to be clarified. Our goal is to examine in vivo reconstitution processes and candidate niches in all bones in the mouse body using a new visualization method. Materials and Methods. Using bone marrow cells from green fluorescent protein (GFP) transgenic mice, the reconstitution processes of transplanted hematopoietic cells (HCs) under myeloablative or nonmyeloablative conditions were observed sequentially from outside the bones with a fluorescent stereomicroscope. Results. In case of myeloablative transplantation, GFP+ spots were first detected at the epiphysis of femurs, and in some ribs and vertebrae among all intact bones. Thereafter, engrafted cells proliferated and spread into other bones. In case of nonmyeloablative transplantation with lin-Sca-1+c-kit+ cells into W/Wv neonates, characterized by vacant niches because of stem cell defects, GFP+ cells localized at the epiphysis of femurs and in some vertebrae and ribs, but not in all bones even 4 months after transplantation. Conclusion. Our findings show that transplanted HSCs or their immature progenies engraft preferentially at the epiphysis of the femurs or short and flat bones such as ribs and vertebrae. The transplanted cells remain quiescent for at least 4 months under nonmyloablative conditions, which implies the presence of stem cells in a niche. Our approach for the first time graphically demonstrates the kinetics of HCs in vivo and should facilitate analysis of HSC behavior in a three-dimensional mode.

Original languageEnglish (US)
Pages (from-to)733-740
Number of pages8
JournalExperimental Hematology
Volume31
Issue number8
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

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Bone and Bones
Epiphyses
Ribs
Hematopoietic Stem Cells
Green Fluorescent Proteins
Femur
Spine
Transplantation
Spatial Behavior
Stem Cell Niche
Bone Marrow Cells
Transgenic Mice
Stem Cells

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Direct visualization of transplanted hematopoietic cellreconstitution in intact mouse organs indicates the presence of a niche. / Yoshimoto, Momoko; Shinohara, Takashi; Heike, Toshio; Shiota, Mitsutaka; Kanatsu-Shinohara, Mito; Nakahata, Tatsutoshi.

In: Experimental Hematology, Vol. 31, No. 8, 01.08.2003, p. 733-740.

Research output: Contribution to journalArticle

Yoshimoto, Momoko ; Shinohara, Takashi ; Heike, Toshio ; Shiota, Mitsutaka ; Kanatsu-Shinohara, Mito ; Nakahata, Tatsutoshi. / Direct visualization of transplanted hematopoietic cellreconstitution in intact mouse organs indicates the presence of a niche. In: Experimental Hematology. 2003 ; Vol. 31, No. 8. pp. 733-740.
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abstract = "Objective. The temporal and spatial behavior of transplanted hematopoietic stem cells (HSCs) within bones remains to be clarified. Our goal is to examine in vivo reconstitution processes and candidate niches in all bones in the mouse body using a new visualization method. Materials and Methods. Using bone marrow cells from green fluorescent protein (GFP) transgenic mice, the reconstitution processes of transplanted hematopoietic cells (HCs) under myeloablative or nonmyeloablative conditions were observed sequentially from outside the bones with a fluorescent stereomicroscope. Results. In case of myeloablative transplantation, GFP+ spots were first detected at the epiphysis of femurs, and in some ribs and vertebrae among all intact bones. Thereafter, engrafted cells proliferated and spread into other bones. In case of nonmyeloablative transplantation with lin-Sca-1+c-kit+ cells into W/Wv neonates, characterized by vacant niches because of stem cell defects, GFP+ cells localized at the epiphysis of femurs and in some vertebrae and ribs, but not in all bones even 4 months after transplantation. Conclusion. Our findings show that transplanted HSCs or their immature progenies engraft preferentially at the epiphysis of the femurs or short and flat bones such as ribs and vertebrae. The transplanted cells remain quiescent for at least 4 months under nonmyloablative conditions, which implies the presence of stem cells in a niche. Our approach for the first time graphically demonstrates the kinetics of HCs in vivo and should facilitate analysis of HSC behavior in a three-dimensional mode.",
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