Discriminatory Indices of Typing Methods for Epidemiologic Analysis of Contemporary Staphylococcus aureus Strains

Marcela Rodriguez, Patrick G. Hogan, Sarah W. Satola, Emily Crispell, Todd Wylie, Hongyu Gao, Erica Sodergren, George M. Weinstock, Carey Ann D. Burnham, Stephanie A. Fritz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Historically, a number of typing methods have been evaluated for Staphylococcus aureus strain characterization. The emergence of contemporary strains of community-associated S. aureus, and the ensuing epidemic with a predominant strain type (USA300), necessitates re-evaluation of the discriminatory power of these typing methods for discerning molecular epidemiology and transmission dynamics, essential to investigations of hospital and community outbreaks. We compared the discriminatory index of 5 typing methods for contemporary S. aureus strain characterization. Children presenting to St. Louis Children's Hospital and community pediatric practices in St. Louis, Missouri (MO), with community-associated S. aureus infections were enrolled. Repetitive sequence-based PCR (repPCR), pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), staphylococcal protein A (spa), and staphylococcal cassette chromosome (SCC) mec typing were performed on 200 S. aureus isolates. The discriminatory index of each method was calculated using the standard formula for this metric, where a value of 1 is highly discriminatory and a value of 0 is not discriminatory. Overall, we identified 26 distinct strain types by repPCR, 17 strain types by PFGE, 30 strain types by MLST, 68 strain types by spa typing, and 5 strain types by SCCmec typing. RepPCR had the highest discriminatory index (D) of all methods (D = 0.88), followed by spa typing (D = 0.87), MLST (D = 0.84), PFGE (D = 0.76), and SCCmec typing (D = 0.60). The method with the highest D among MRSA isolates was repPCR (D = 0.64) followed by spa typing (D = 0.45) and MLST (D = 0.44). The method with the highest D among MSSA isolates was spa typing (D = 0.98), followed by MLST (D = 0.93), repPCR (D = 0.92), and PFGE (D = 0.89). Among isolates designated USA300 by PFGE, repPCR was most discriminatory, with 10 distinct strain types identified (D = 0.63). We identified 45 MRSA isolates which were classified as identical by PFGE, MLST, spa typing, and SCCmec typing (USA300, ST8, t008, SCCmec IV, respectively); within this collection, there were 5 distinct strain types identified by repPCR. The typing methods yielded comparable discriminatory power for S. aureus characterization overall; when discriminating among USA300 isolates, repPCR retained the highest discriminatory power. This property is advantageous for investigations conducted in the era of contemporary S. aureus infections.

Original languageEnglish (US)
Pages (from-to)e1534
JournalMedicine
Volume94
Issue number37
DOIs
StatePublished - Sep 1 2015
Externally publishedYes

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Epidemiologic Methods
Multilocus Sequence Typing
Staphylococcus aureus
Pulsed Field Gel Electrophoresis
Staphylococcal Protein A
Methicillin-Resistant Staphylococcus aureus
Pediatric Hospitals
Molecular Epidemiology
Nucleic Acid Repetitive Sequences
Infection
Disease Outbreaks
Chromosomes
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Rodriguez, M., Hogan, P. G., Satola, S. W., Crispell, E., Wylie, T., Gao, H., ... Fritz, S. A. (2015). Discriminatory Indices of Typing Methods for Epidemiologic Analysis of Contemporary Staphylococcus aureus Strains. Medicine, 94(37), e1534. https://doi.org/10.1097/MD.0000000000001534

Discriminatory Indices of Typing Methods for Epidemiologic Analysis of Contemporary Staphylococcus aureus Strains. / Rodriguez, Marcela; Hogan, Patrick G.; Satola, Sarah W.; Crispell, Emily; Wylie, Todd; Gao, Hongyu; Sodergren, Erica; Weinstock, George M.; Burnham, Carey Ann D.; Fritz, Stephanie A.

In: Medicine, Vol. 94, No. 37, 01.09.2015, p. e1534.

Research output: Contribution to journalArticle

Rodriguez, M, Hogan, PG, Satola, SW, Crispell, E, Wylie, T, Gao, H, Sodergren, E, Weinstock, GM, Burnham, CAD & Fritz, SA 2015, 'Discriminatory Indices of Typing Methods for Epidemiologic Analysis of Contemporary Staphylococcus aureus Strains', Medicine, vol. 94, no. 37, pp. e1534. https://doi.org/10.1097/MD.0000000000001534
Rodriguez, Marcela ; Hogan, Patrick G. ; Satola, Sarah W. ; Crispell, Emily ; Wylie, Todd ; Gao, Hongyu ; Sodergren, Erica ; Weinstock, George M. ; Burnham, Carey Ann D. ; Fritz, Stephanie A. / Discriminatory Indices of Typing Methods for Epidemiologic Analysis of Contemporary Staphylococcus aureus Strains. In: Medicine. 2015 ; Vol. 94, No. 37. pp. e1534.
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