Disruption of MHC class II-restricted antigen presentation by vaccinia virus

Ping Li, Nan Wang, Delu Zhou, Christina S K Yee, Cheong Hee Chang, Randy Brutkiewicz, Janice Blum

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Abstract

Vaccinia virus (VV), currently used in humans as a live vaccine for smallpox, can interfere with host immunity via several discrete mechanisms. In this study, the effect of VV on MHC class II-mediated Ag presentation was investigated. Following VV infection, the ability of professional and nonprofessional APC to present Ag and peptides to CD4+ T cells was impaired. Viral inhibition of class II Ag presentation could be detected within 1 h, with diminished T cell responses dependent upon the duration of APC infection and virus titer. Exposure of APC to replication-deficient virus also diminished class II Ag presentation. Virus infection of APC perturbed Ag presentation by newly synthesized and recycling class II molecules, with disruptions in both exogenous and cytoplasmic Ag presentation. Virus-driven expression of an endogenous Ag, failed to restore T cell responsiveness specific for this Ag in the context of MHC class II molecules. Yet, both class II protein steady-state and cell surface expression were not altered by VV. Biochemical and functional analysis revealed that VV infection directly interfered with ligand binding to class II molecules. Together, these observations suggest that disruption of MHC class II-mediated Ag presentation may be one of multiple strategies VV has evolved to escape host immune surveillance.

Original languageEnglish
Pages (from-to)6481-6488
Number of pages8
JournalJournal of Immunology
Volume175
Issue number10
StatePublished - Nov 15 2005

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Vaccinia virus
Histocompatibility Antigens Class II
Antigen Presentation
Virus Diseases
T-Lymphocytes
Smallpox Vaccine
Human Adenoviruses
Recycling
Virus Replication
Viral Load
Immunity
Ligands
Viruses
Peptides
Infection
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Li, P., Wang, N., Zhou, D., Yee, C. S. K., Chang, C. H., Brutkiewicz, R., & Blum, J. (2005). Disruption of MHC class II-restricted antigen presentation by vaccinia virus. Journal of Immunology, 175(10), 6481-6488.

Disruption of MHC class II-restricted antigen presentation by vaccinia virus. / Li, Ping; Wang, Nan; Zhou, Delu; Yee, Christina S K; Chang, Cheong Hee; Brutkiewicz, Randy; Blum, Janice.

In: Journal of Immunology, Vol. 175, No. 10, 15.11.2005, p. 6481-6488.

Research output: Contribution to journalArticle

Li, P, Wang, N, Zhou, D, Yee, CSK, Chang, CH, Brutkiewicz, R & Blum, J 2005, 'Disruption of MHC class II-restricted antigen presentation by vaccinia virus', Journal of Immunology, vol. 175, no. 10, pp. 6481-6488.
Li P, Wang N, Zhou D, Yee CSK, Chang CH, Brutkiewicz R et al. Disruption of MHC class II-restricted antigen presentation by vaccinia virus. Journal of Immunology. 2005 Nov 15;175(10):6481-6488.
Li, Ping ; Wang, Nan ; Zhou, Delu ; Yee, Christina S K ; Chang, Cheong Hee ; Brutkiewicz, Randy ; Blum, Janice. / Disruption of MHC class II-restricted antigen presentation by vaccinia virus. In: Journal of Immunology. 2005 ; Vol. 175, No. 10. pp. 6481-6488.
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