Dissociation of colonic apical Na/H exchange activity from bulk cytoplasmic pH

Pierre C. Dagher, Thomas Behm, Angela Taglietta-Kohlbrecher, Richard W. Egnor, Alan N. Charney

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17 Scopus citations

Abstract

Intracellular acidification by stimuli other than CO2 fails to stimulate colonic apical Na/H exchange and Na absorption. We examined whether Na absorption could be stimulated in the absence of changes in cytoplasmic pH (pH(i)). Distal colon of male Sprague-Dawley rats was used for pH(i) measurements with 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein and for flux measurements in Ussing chambers. In 21 mM HCO3-Ringer, increasing PCO2 from 20 to 70 mmHg decreased pH(i) from 7.51 to 7.03 and increased net Na flux (J(net)/(Na)) from 4.2 ± 0.4 to 6.8 ± 0.6 μeq · cm-2 · h-1. Similar increases in J(net)/(Na) occurred in the absence of mucosal Cl and in the presence of phalloidin to inhibit microfilaments or benzolamide to inhibit membrane-bound carbonic anhydrase. Isohydric increases in PCO2 did not alter pH(i) but stimulated J(net)/(Na) from 5.1 ± 0.6 to 7.2 ± 0.8 μeq · cm-2 · h-1. Carbonyl cyanide m-chlorophenylhydrazone (CCCP) decreased pH(i) from 7.45 to 7.35 but did not stimulate J(net)/(Na). Butyrate (25 mM) decreased pH(i) from 7.15 to 7.02 with recovery to baseline within 6 min; however, J(net)/(Na) increased by 2.2 μeq · cm-2 · h-1 for 60 min. We conclude that apical Na/H exchange activity is unresponsive to changes in bulk pH(i) and is independent of Cl-/HCO3 exchange, microfilaments, and membrane-bound carbonic anhydrase. The presence of an H-tight, CO2, and butyrate-permeable subapical domain is postulated.

Original languageEnglish (US)
Pages (from-to)C1799-C1806
JournalAmerican Journal of Physiology - Cell Physiology
Volume270
Issue number6 39-6
StatePublished - Jun 1 1996

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Keywords

  • 2',7'-bis(carboxyethyl)-5(6)- carboxyfluorescein
  • Sprague-Dawley rat
  • carbonic anhydrase
  • intracellular pH
  • sodium absorption

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Dagher, P. C., Behm, T., Taglietta-Kohlbrecher, A., Egnor, R. W., & Charney, A. N. (1996). Dissociation of colonic apical Na/H exchange activity from bulk cytoplasmic pH. American Journal of Physiology - Cell Physiology, 270(6 39-6), C1799-C1806.