Distal urinary acidification from Homer Smith to the present

Stephen L. Gluck, Masahiro Iyori, L. Shannon Holliday, Tatiana Kostrominova, Beth S. Lee

Research output: Contribution to journalArticle

18 Scopus citations


Since Smith's time, the essential role of collecting duct intercalated cells in controlling net acid excretion has been recognized. Rather than employing an H+-exchange mechanism, intercalated cells have V-ATPase on the plasma membrane and in plasmalemma-associated tubulovesicles, which functions in the bicarbonate reabsorption, regeneration, and bicarbonate secretion required for acid-base homeostasis. Several distinct mechanisms participate in regulating V-ATpase-driven H+ secretion in different cell types: (1) Renal epithelial cells have the capacity to express different structural forms of V-ATPase that have intrinsic differences in their enzymatic properties. 2) The kidney produces cytosolic regulatory proteins, capable of interacting directly with the V-ATPase, that may modify its activity. V-ATPases in different cell types may differ in the degree to which their activity is affected by regulatory factors, as a result of variations in V-ATPase structure. (3) In the α intercalated cell, the number of active V-ATPases on the luminal membrane is controlled in vivo by membrane vesicle-mediated traffic that may require unidentified mediators. In the β intercalated cell, the number of active V-ATPases on the basolateral membrane may be controlled by regulated assembly and disassembly, responding directly to extracellular pH.

Original languageEnglish (US)
Pages (from-to)1660-1664
Number of pages5
JournalKidney international
Issue number6
StatePublished - Jan 1 1996
Externally publishedYes

ASJC Scopus subject areas

  • Nephrology

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