Distinct endosomal compartments in early trafficking of low density lipoprotein-derived cholesterol

Shigeki Sugii, Patrick C. Reid, Nobutaka Ohgami, Hong Du, Ta Yuan Chang

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

We previously studied the early trafficking of low density lipoprotein (LDL)-derived cholesterol in mutant Chinese hamster ovary cells defective in Niemann-Pick type C1 (NPC1) using cyclodextrin (CD) to monitor the arrival of cholesterol from the cell interior to the plasma membrane (PM) (Cruz, J. C., Sugii, S., Yu, C., and Chang, T.-Y. (2000) J. Biol. Chem. 275, 4013-4021). We found that newly hydrolyzed cholesterol derived from LDL first appears in certain CD-accessible pool(s), which we assumed to be the PM, before accumulating in the late endosome/lysosome, where NPC1 resides. To determine the identity of the early CD-accessible pool(s), in this study, we performed additional experiments, including the use of revised CD incubation protocols. We found that prolonged incubation with CD (>30 min) caused cholesterol in internal membrane compartment(s) to redistribute to the PM, where it became accessible to CD. In contrast, a short incubation with CD (5-10 min) did not cause such an effect. We also show that one of the early compartments contains acid lipase (AL), the enzyme required for liberating cholesterol from cholesteryl ester in LDL. Biochemical and microscopic evidence indicates that most of the AL is present in endocytic compartment(s) distinct from the late endosome/lysosome. Our results suggest that cholesterol is liberated from LDL cholesteryl ester in the hydrolytic compartment containing AL and then moves to the NPC1-containing late endosome/lysosome before reaching the PM or the endoplasmic reticulum.

Original languageEnglish (US)
Pages (from-to)27180-27189
Number of pages10
JournalJournal of Biological Chemistry
Volume278
Issue number29
DOIs
StatePublished - Jul 18 2003
Externally publishedYes

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Cyclodextrins
LDL Lipoproteins
LDL Cholesterol
Cholesterol
Cell membranes
Cholesterol Esters
Endosomes
Lysosomes
Lipase
Cell Membrane
Cricetulus
Endoplasmic Reticulum
Ovary
Cells
Membranes
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Distinct endosomal compartments in early trafficking of low density lipoprotein-derived cholesterol. / Sugii, Shigeki; Reid, Patrick C.; Ohgami, Nobutaka; Du, Hong; Chang, Ta Yuan.

In: Journal of Biological Chemistry, Vol. 278, No. 29, 18.07.2003, p. 27180-27189.

Research output: Contribution to journalArticle

Sugii, Shigeki ; Reid, Patrick C. ; Ohgami, Nobutaka ; Du, Hong ; Chang, Ta Yuan. / Distinct endosomal compartments in early trafficking of low density lipoprotein-derived cholesterol. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 29. pp. 27180-27189.
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