Distinct patterns of expression of the RB gene family in mouse and human retina

Clarellen Spencer, Sanja Pajovic, Hollie Devlin, Quynh Dao Dinh, Timothy Corson, Brenda L. Gallie

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Although RB1 function is disrupted in the majority of human cancers, an undefined cell of developing human retina is uniquely sensitive to cancer induction when the RB1 tumor suppressor gene is lost. Murine retinoblastoma is initiated only when two of the RB family of genes, RB1 and p107 or p130, are inactivated. Although whole embryonic retina shows RB family gene expression by several techniques, when E14 developing retina was depleted of the earliest differentiating cells, ganglion cells, the remaining proliferating murine embryonic retinal progenitor cells clearly did not express RB1 or p130, while the longer splice form of p107 was expressed. Each retinal cell type expressed some member of the RB family at some stage of differentiation. Rod photoreceptors stained for the RB1 protein product, pRB, and p107 in only a brief window of postnatal murine development, with no detectable staining for any of the RB family proteins in adult human and mouse rod photoreceptors. Adult mouse and human Müller glia, ganglion and rare horizontal cells, and adult human, but not adult mouse, cone photoreceptors stained for pRB. The RB gene family is dynamically and variably expressed through retinal development in specific retinal cells.

Original languageEnglish (US)
Pages (from-to)687-694
Number of pages8
JournalGene Expression Patterns
Volume5
Issue number5
DOIs
StatePublished - Jun 2005
Externally publishedYes

Fingerprint

Retina
Gene Expression
Retinal Rod Photoreceptor Cells
Ganglia
Retinal Cone Photoreceptor Cells
Retinoblastoma
Tumor Suppressor Genes
Neuroglia
Genes
Neoplasms
Proteins
Stem Cells
Staining and Labeling

Keywords

  • Cone photoreceptors
  • Ganglion cells
  • Gene expression
  • Horizontal cells
  • Immunohistochemistry
  • Müller glia cells
  • Murine retinal development
  • p107
  • p130
  • RB1 gene and protein
  • RB1 mice
  • Retinoblastoma
  • Rod photoreceptors

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Distinct patterns of expression of the RB gene family in mouse and human retina. / Spencer, Clarellen; Pajovic, Sanja; Devlin, Hollie; Dinh, Quynh Dao; Corson, Timothy; Gallie, Brenda L.

In: Gene Expression Patterns, Vol. 5, No. 5, 06.2005, p. 687-694.

Research output: Contribution to journalArticle

Spencer, Clarellen ; Pajovic, Sanja ; Devlin, Hollie ; Dinh, Quynh Dao ; Corson, Timothy ; Gallie, Brenda L. / Distinct patterns of expression of the RB gene family in mouse and human retina. In: Gene Expression Patterns. 2005 ; Vol. 5, No. 5. pp. 687-694.
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