Distinct patterns of gene expression in the skin lesions of atopic dermatitis and psoriasis: A gene microarray analysis

Ichiro Nomura, Bifeng Gao, Mark Boguniewicz, Marc A. Darst, Jeffrey Travers, Donald Y M Leung

Research output: Contribution to journalArticle

247 Citations (Scopus)

Abstract

Background: Atopic dermatitis (AD) and psoriasis are the two most common chronic inflammatory skin diseases. Both of these diseases have distinct clinical findings and specific inflammatory cell infiltrates. Previous reports have focused individually on one or two genes or gene products in the lesions of both skin diseases. However, they have not captured the complex gene expression that must occur to induce specific cellular infiltrates in the skin lesions of these two diseases. DNA microarray studies allow the simultaneous comparison of thousands of messenger RNAs that may identify the disease-specific pattern of tissue inflammatory responses. Objective: To compare the complex gene expression pattern of AD versus psoriasis skin lesions. Methods: RNA was extracted from skin biopsy specimens of 6 patients with AD and 7 patients with psoriasis and analyzed with the use of Hu-U95Av.GeneChip microarrays. To confirm GeneChip results, real-time PCR of selected genes were performed. Results: In AD skin, a total of 18 genes including the CC chemokines, CCL-13/MCP-4, CCL-18/PARC, and CCL-27/CTACK showed a statistically significant, >2-fold increase of gene expression compared with psoriasis. In psoriasis skin, a total of 62 genes including CCL-4/MIP-1β, CCL-20/MIP-3α, CXCL-2/GRO-β CXCL-8/IL-8, and CXCR2/IL-8R showed a >2-fold increase of gene expression compared with AD skin. Real-time PCR confirmed several of these GeneChip results. Conclusions: These results show a very distinctive gene expression pattern in AD as compared with psoriasis that may explain several features of AD and psoriasis including the specific inflammatory cell infiltrates observed in these disorders, that is, TH2 cells, eosinophils, and mast cells in AD and TH1 cells and neutrophils in psoriasis. Such observations may contribute to a characteristic "signature" for these two skin diseases.

Original languageEnglish
Pages (from-to)1195-1202
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Volume112
Issue number6
DOIs
StatePublished - Dec 2003

Fingerprint

Atopic Dermatitis
Microarray Analysis
Psoriasis
Gene Expression
Skin
Genes
Skin Diseases
Real-Time Polymerase Chain Reaction
CC Chemokines
Oligonucleotide Array Sequence Analysis
Interleukin-8
Eosinophils
Mast Cells
Neutrophils
RNA
Biopsy
Messenger RNA

Keywords

  • Atopic dermatitis
  • Chemokines
  • Genes
  • Psoriasis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Distinct patterns of gene expression in the skin lesions of atopic dermatitis and psoriasis : A gene microarray analysis. / Nomura, Ichiro; Gao, Bifeng; Boguniewicz, Mark; Darst, Marc A.; Travers, Jeffrey; Leung, Donald Y M.

In: Journal of Allergy and Clinical Immunology, Vol. 112, No. 6, 12.2003, p. 1195-1202.

Research output: Contribution to journalArticle

Nomura, Ichiro ; Gao, Bifeng ; Boguniewicz, Mark ; Darst, Marc A. ; Travers, Jeffrey ; Leung, Donald Y M. / Distinct patterns of gene expression in the skin lesions of atopic dermatitis and psoriasis : A gene microarray analysis. In: Journal of Allergy and Clinical Immunology. 2003 ; Vol. 112, No. 6. pp. 1195-1202.
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abstract = "Background: Atopic dermatitis (AD) and psoriasis are the two most common chronic inflammatory skin diseases. Both of these diseases have distinct clinical findings and specific inflammatory cell infiltrates. Previous reports have focused individually on one or two genes or gene products in the lesions of both skin diseases. However, they have not captured the complex gene expression that must occur to induce specific cellular infiltrates in the skin lesions of these two diseases. DNA microarray studies allow the simultaneous comparison of thousands of messenger RNAs that may identify the disease-specific pattern of tissue inflammatory responses. Objective: To compare the complex gene expression pattern of AD versus psoriasis skin lesions. Methods: RNA was extracted from skin biopsy specimens of 6 patients with AD and 7 patients with psoriasis and analyzed with the use of Hu-U95Av.GeneChip microarrays. To confirm GeneChip results, real-time PCR of selected genes were performed. Results: In AD skin, a total of 18 genes including the CC chemokines, CCL-13/MCP-4, CCL-18/PARC, and CCL-27/CTACK showed a statistically significant, >2-fold increase of gene expression compared with psoriasis. In psoriasis skin, a total of 62 genes including CCL-4/MIP-1β, CCL-20/MIP-3α, CXCL-2/GRO-β CXCL-8/IL-8, and CXCR2/IL-8R showed a >2-fold increase of gene expression compared with AD skin. Real-time PCR confirmed several of these GeneChip results. Conclusions: These results show a very distinctive gene expression pattern in AD as compared with psoriasis that may explain several features of AD and psoriasis including the specific inflammatory cell infiltrates observed in these disorders, that is, TH2 cells, eosinophils, and mast cells in AD and TH1 cells and neutrophils in psoriasis. Such observations may contribute to a characteristic {"}signature{"} for these two skin diseases.",
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AU - Nomura, Ichiro

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AU - Darst, Marc A.

AU - Travers, Jeffrey

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AB - Background: Atopic dermatitis (AD) and psoriasis are the two most common chronic inflammatory skin diseases. Both of these diseases have distinct clinical findings and specific inflammatory cell infiltrates. Previous reports have focused individually on one or two genes or gene products in the lesions of both skin diseases. However, they have not captured the complex gene expression that must occur to induce specific cellular infiltrates in the skin lesions of these two diseases. DNA microarray studies allow the simultaneous comparison of thousands of messenger RNAs that may identify the disease-specific pattern of tissue inflammatory responses. Objective: To compare the complex gene expression pattern of AD versus psoriasis skin lesions. Methods: RNA was extracted from skin biopsy specimens of 6 patients with AD and 7 patients with psoriasis and analyzed with the use of Hu-U95Av.GeneChip microarrays. To confirm GeneChip results, real-time PCR of selected genes were performed. Results: In AD skin, a total of 18 genes including the CC chemokines, CCL-13/MCP-4, CCL-18/PARC, and CCL-27/CTACK showed a statistically significant, >2-fold increase of gene expression compared with psoriasis. In psoriasis skin, a total of 62 genes including CCL-4/MIP-1β, CCL-20/MIP-3α, CXCL-2/GRO-β CXCL-8/IL-8, and CXCR2/IL-8R showed a >2-fold increase of gene expression compared with AD skin. Real-time PCR confirmed several of these GeneChip results. Conclusions: These results show a very distinctive gene expression pattern in AD as compared with psoriasis that may explain several features of AD and psoriasis including the specific inflammatory cell infiltrates observed in these disorders, that is, TH2 cells, eosinophils, and mast cells in AD and TH1 cells and neutrophils in psoriasis. Such observations may contribute to a characteristic "signature" for these two skin diseases.

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