Distinct repriming and closed-state inactivation kinetics of Nav1.6 and Nav1.7 sodium channels in mouse spinal sensory neurons

Raimund I. Herzog, Theodore Cummins, Farshid Ghassemi, Sulayman D. Dib-Hajj, Stephen G. Waxman

Research output: Contribution to journalArticle

216 Citations (Scopus)

Abstract

While large, myelinated dorsal root ganglion (DRG) neurons are capable of firing at high frequencies, small unmyelinated DRG neurons typically display much lower maximum firing frequencies. However, the molecular basis for this difference has not been delineated. Because the sodium currents in large DRG neurons exhibit rapid repriming (recovery from inactivation) kinetics and the sodium currents in small DRG neurons exhibit predominantly slow repriming kinetics, it has been proposed that differences in sodium channels might contribute to the determination of repetitive firing properties in DRG neurons. A recent study demonstrated that Nav1.7 expression is negatively correlated with conduction velocity and DRG cell size, while the Nav1.6 voltage-gated sodium channel has been implicated as the predominant isoform present at nodes of Ranvier of myelinated fibres. Therefore we characterized and compared the functional properties, including repriming, of recombinant Nav1.6 and Nav1.7 channels expressed in mouse DRG neurons. Both Nav1.6 and Nav1.7 channels generated fast-activating and fast-inactivating currents. However recovery from inactivation was significantly faster (∼-5-fold at -70 mV) for Nav1.6 currents than for Nav1.7 currents. The recovery from inactivation of Nav1.6 channels was also much faster than that of native tetrodotoxin-sensitive sodium currents recorded from small spinal sensory neurons, but similar to that of tetrodotoxin-sensitive sodium currents recorded from large spinal sensory neurons. Development of closed-state inactivation was also much faster for Nav1.6 currents than for Nav1.7 currents. Our results indicate that the firing properties of DRG neurons can be tuned by regulating expression of different sodium channel isoforms that have distinct repriming and closed-state inactivation kinetics.

Original languageEnglish (US)
Pages (from-to)741-750
Number of pages10
JournalJournal of Physiology
Volume551
Issue number3
DOIs
StatePublished - Sep 15 2003
Externally publishedYes

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Sodium Channels
Spinal Ganglia
Sensory Receptor Cells
Neurons
Sodium
Tetrodotoxin
NAV1.6 Voltage-Gated Sodium Channel
Protein Isoforms
Ranvier's Nodes
Cell Size

ASJC Scopus subject areas

  • Physiology

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Distinct repriming and closed-state inactivation kinetics of Nav1.6 and Nav1.7 sodium channels in mouse spinal sensory neurons. / Herzog, Raimund I.; Cummins, Theodore; Ghassemi, Farshid; Dib-Hajj, Sulayman D.; Waxman, Stephen G.

In: Journal of Physiology, Vol. 551, No. 3, 15.09.2003, p. 741-750.

Research output: Contribution to journalArticle

Herzog, Raimund I. ; Cummins, Theodore ; Ghassemi, Farshid ; Dib-Hajj, Sulayman D. ; Waxman, Stephen G. / Distinct repriming and closed-state inactivation kinetics of Nav1.6 and Nav1.7 sodium channels in mouse spinal sensory neurons. In: Journal of Physiology. 2003 ; Vol. 551, No. 3. pp. 741-750.
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