Distribution of marrow repopulating cells between bone marrow and spleen early after transplantation

P. Artur Plett, Stacy M. Frankovitz, Christie M. Orschell

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Whether hematopoietic stem cells (HSCs) home selectively to bone marrow (BM) early after transplantation remains an issue of debate. Better understanding of homing mechanisms may benefit BM transplantation protocols in cases of limited graft cell number or nonmyeloablative conditioning regimens. Using flow cytometry and serial transplantation to stringently identify HSCs, trafficking patterns of long-term engrafting cells were mapped between BM and spleen early after transplantation. Low-density BM cells were tracked in irradiated or nonirradiated mice 1, 3, 6, and 20 hours after transplantation, at which time recipient BM and spleen were analyzed for recovery of primitive donor cells by phenotype and adhesion molecule expression. In addition, phenotypically defined HSC-enriched or HSC-depleted grafts were tracked 20 hours after transplantation in recipient BM and spleen and analyzed for recovery and long-term repopulating potential in mice undergoing serial transplantation. Regardless of irradiation status, recovery of donor Sca-1 + lin- cells was higher at most time points in recipient BM than in spleen, while recovery of total Sca-1+ cells was variable. A significantly higher percentage of BM-homed donor Sca-1+ cells expressed CD43, CD49e, and CD49d 20 hours after transplantation than spleen-homed cells, which contained significantly more non-HSC phenotypes. Furthermore, BM-homed cells were significantly enriched for cells capable of secondary multilineage hematopoiesis in mice undergoing serial transplantation compared with spleen-homed cells. These results support the notion of specific homing of HSCs to BM by 20 hours after transplantation and provide a basis for the enhanced engraftment potential afforded some Sca-1+ lin - cells subfractionated on the basis of adhesion molecule expression.

Original languageEnglish (US)
Pages (from-to)2285-2291
Number of pages7
JournalBlood
Volume102
Issue number6
DOIs
StatePublished - Sep 15 2003

Fingerprint

Bone Marrow Cells
Bone
Spleen
Transplantation
Bone Marrow
Stem cells
Hematopoietic Stem Cells
Transplantation (surgical)
Recovery
Grafts
Adhesion
Transplants
Phenotype
Hematopoiesis
Molecules
Cell Adhesion Molecules
Flow cytometry
Bone Marrow Transplantation
Flow Cytometry
Stem Cells

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Distribution of marrow repopulating cells between bone marrow and spleen early after transplantation. / Plett, P. Artur; Frankovitz, Stacy M.; Orschell, Christie M.

In: Blood, Vol. 102, No. 6, 15.09.2003, p. 2285-2291.

Research output: Contribution to journalArticle

Plett, P. Artur ; Frankovitz, Stacy M. ; Orschell, Christie M. / Distribution of marrow repopulating cells between bone marrow and spleen early after transplantation. In: Blood. 2003 ; Vol. 102, No. 6. pp. 2285-2291.
@article{624182b89ec54fefb021c541fb239824,
title = "Distribution of marrow repopulating cells between bone marrow and spleen early after transplantation",
abstract = "Whether hematopoietic stem cells (HSCs) home selectively to bone marrow (BM) early after transplantation remains an issue of debate. Better understanding of homing mechanisms may benefit BM transplantation protocols in cases of limited graft cell number or nonmyeloablative conditioning regimens. Using flow cytometry and serial transplantation to stringently identify HSCs, trafficking patterns of long-term engrafting cells were mapped between BM and spleen early after transplantation. Low-density BM cells were tracked in irradiated or nonirradiated mice 1, 3, 6, and 20 hours after transplantation, at which time recipient BM and spleen were analyzed for recovery of primitive donor cells by phenotype and adhesion molecule expression. In addition, phenotypically defined HSC-enriched or HSC-depleted grafts were tracked 20 hours after transplantation in recipient BM and spleen and analyzed for recovery and long-term repopulating potential in mice undergoing serial transplantation. Regardless of irradiation status, recovery of donor Sca-1 + lin- cells was higher at most time points in recipient BM than in spleen, while recovery of total Sca-1+ cells was variable. A significantly higher percentage of BM-homed donor Sca-1+ cells expressed CD43, CD49e, and CD49d 20 hours after transplantation than spleen-homed cells, which contained significantly more non-HSC phenotypes. Furthermore, BM-homed cells were significantly enriched for cells capable of secondary multilineage hematopoiesis in mice undergoing serial transplantation compared with spleen-homed cells. These results support the notion of specific homing of HSCs to BM by 20 hours after transplantation and provide a basis for the enhanced engraftment potential afforded some Sca-1+ lin - cells subfractionated on the basis of adhesion molecule expression.",
author = "Plett, {P. Artur} and Frankovitz, {Stacy M.} and Orschell, {Christie M.}",
year = "2003",
month = "9",
day = "15",
doi = "10.1182/blood-2002-12-3742",
language = "English (US)",
volume = "102",
pages = "2285--2291",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "6",

}

TY - JOUR

T1 - Distribution of marrow repopulating cells between bone marrow and spleen early after transplantation

AU - Plett, P. Artur

AU - Frankovitz, Stacy M.

AU - Orschell, Christie M.

PY - 2003/9/15

Y1 - 2003/9/15

N2 - Whether hematopoietic stem cells (HSCs) home selectively to bone marrow (BM) early after transplantation remains an issue of debate. Better understanding of homing mechanisms may benefit BM transplantation protocols in cases of limited graft cell number or nonmyeloablative conditioning regimens. Using flow cytometry and serial transplantation to stringently identify HSCs, trafficking patterns of long-term engrafting cells were mapped between BM and spleen early after transplantation. Low-density BM cells were tracked in irradiated or nonirradiated mice 1, 3, 6, and 20 hours after transplantation, at which time recipient BM and spleen were analyzed for recovery of primitive donor cells by phenotype and adhesion molecule expression. In addition, phenotypically defined HSC-enriched or HSC-depleted grafts were tracked 20 hours after transplantation in recipient BM and spleen and analyzed for recovery and long-term repopulating potential in mice undergoing serial transplantation. Regardless of irradiation status, recovery of donor Sca-1 + lin- cells was higher at most time points in recipient BM than in spleen, while recovery of total Sca-1+ cells was variable. A significantly higher percentage of BM-homed donor Sca-1+ cells expressed CD43, CD49e, and CD49d 20 hours after transplantation than spleen-homed cells, which contained significantly more non-HSC phenotypes. Furthermore, BM-homed cells were significantly enriched for cells capable of secondary multilineage hematopoiesis in mice undergoing serial transplantation compared with spleen-homed cells. These results support the notion of specific homing of HSCs to BM by 20 hours after transplantation and provide a basis for the enhanced engraftment potential afforded some Sca-1+ lin - cells subfractionated on the basis of adhesion molecule expression.

AB - Whether hematopoietic stem cells (HSCs) home selectively to bone marrow (BM) early after transplantation remains an issue of debate. Better understanding of homing mechanisms may benefit BM transplantation protocols in cases of limited graft cell number or nonmyeloablative conditioning regimens. Using flow cytometry and serial transplantation to stringently identify HSCs, trafficking patterns of long-term engrafting cells were mapped between BM and spleen early after transplantation. Low-density BM cells were tracked in irradiated or nonirradiated mice 1, 3, 6, and 20 hours after transplantation, at which time recipient BM and spleen were analyzed for recovery of primitive donor cells by phenotype and adhesion molecule expression. In addition, phenotypically defined HSC-enriched or HSC-depleted grafts were tracked 20 hours after transplantation in recipient BM and spleen and analyzed for recovery and long-term repopulating potential in mice undergoing serial transplantation. Regardless of irradiation status, recovery of donor Sca-1 + lin- cells was higher at most time points in recipient BM than in spleen, while recovery of total Sca-1+ cells was variable. A significantly higher percentage of BM-homed donor Sca-1+ cells expressed CD43, CD49e, and CD49d 20 hours after transplantation than spleen-homed cells, which contained significantly more non-HSC phenotypes. Furthermore, BM-homed cells were significantly enriched for cells capable of secondary multilineage hematopoiesis in mice undergoing serial transplantation compared with spleen-homed cells. These results support the notion of specific homing of HSCs to BM by 20 hours after transplantation and provide a basis for the enhanced engraftment potential afforded some Sca-1+ lin - cells subfractionated on the basis of adhesion molecule expression.

UR - http://www.scopus.com/inward/record.url?scp=0141567531&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141567531&partnerID=8YFLogxK

U2 - 10.1182/blood-2002-12-3742

DO - 10.1182/blood-2002-12-3742

M3 - Article

C2 - 12775569

AN - SCOPUS:0141567531

VL - 102

SP - 2285

EP - 2291

JO - Blood

JF - Blood

SN - 0006-4971

IS - 6

ER -