Distribution of Three Cariogenic Bacteria in Secondary Carious Lesions around Amalgam Restorations

Carlos González-Cabezas, Y. Li, Richard Gregory, G. K. Stookey

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Secondary dental caries remains an unresolved problem in dentistry and little is known of its microbial etiology. The purpose of this study was to compare the distribution of the three most suspected cariogenic groups of bacteria, mutans streptococci. Actinomyces naeslundii genospecies 2 and lactobacilli, in natural secondary caries around amalgam restorations. Extracted teeth with secondary caries were sectioned to obtain three samples that were randomly distributed to three different groups. Each group was immunolabeled with antibodies to either Streptococcus mutans, A. naeslundii genospecies 2 or Lactobacillus casei and subsequently labeled with secondary fluorescent antibodies. All samples were analyzed three-dimensionally using confocal microscopy. The results indicated that the three different bacteria were widely present and could have an important role in the development of secondary caries around amalgam restorations.

Original languageEnglish
Pages (from-to)357-365
Number of pages9
JournalCaries Research
Volume33
Issue number5
DOIs
StatePublished - Sep 1999

Fingerprint

Streptococcus mutans
Bacteria
Lactobacillus casei
Actinomyces
Antibodies
Dental Caries
Lactobacillus
Dentistry
Confocal Microscopy
Tooth

Keywords

  • Actinomyces naeslundii
  • Amalgam
  • Confocal microscopy
  • Lactobacilli mutans streptococci
  • Secondary caries

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Distribution of Three Cariogenic Bacteria in Secondary Carious Lesions around Amalgam Restorations. / González-Cabezas, Carlos; Li, Y.; Gregory, Richard; Stookey, G. K.

In: Caries Research, Vol. 33, No. 5, 09.1999, p. 357-365.

Research output: Contribution to journalArticle

González-Cabezas, Carlos ; Li, Y. ; Gregory, Richard ; Stookey, G. K. / Distribution of Three Cariogenic Bacteria in Secondary Carious Lesions around Amalgam Restorations. In: Caries Research. 1999 ; Vol. 33, No. 5. pp. 357-365.
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