Disturbances in the murine hepatic circadian clock in alcohol-induced hepatic steatosis

Peng Zhou, Ruth A. Ross, Cameron M. Pywell, Suthat Liangpunsakul, Giles E. Duffield

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

To investigate the role of the circadian clock in the development of alcohol-induced fatty liver disease we examined livers of mice chronically alcohol-fed over 4-weeks that resulted in steatosis. Here we show time-of-day specific changes in expression of clock genes and clock-controlled genes, including those associated with lipid and bile acid regulation. Such changes were not observed following a 1-week alcohol treatment with no hepatic lipid accumulation. Real-time bioluminescence reporting of PERIOD2 protein expression suggests that these changes occur independently of the suprachiasmatic nucleus pacemaker. Further, we find profound time-of-day specific changes to the rhythmic synthesis/accumulation of triglycerides, cholesterol and bile acid, and the NAD/NADH ratio, processes that are under clock control. These results highlight not only that the circadian timekeeping system is disturbed in the alcohol-induced hepatic steatosis state, but also that the effects of alcohol upon the clock itself may actually contribute to the development of hepatic steatosis.

Original languageEnglish
Article number03725
JournalScientific Reports
Volume4
DOIs
StatePublished - Jan 16 2014

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Circadian Clocks
Alcohols
Liver
Bile Acids and Salts
NAD
Lipids
Suprachiasmatic Nucleus
Fatty Liver
Liver Diseases
Triglycerides
Cholesterol
Gene Expression
Genes
Proteins

ASJC Scopus subject areas

  • General

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Disturbances in the murine hepatic circadian clock in alcohol-induced hepatic steatosis. / Zhou, Peng; Ross, Ruth A.; Pywell, Cameron M.; Liangpunsakul, Suthat; Duffield, Giles E.

In: Scientific Reports, Vol. 4, 03725, 16.01.2014.

Research output: Contribution to journalArticle

Zhou, Peng ; Ross, Ruth A. ; Pywell, Cameron M. ; Liangpunsakul, Suthat ; Duffield, Giles E. / Disturbances in the murine hepatic circadian clock in alcohol-induced hepatic steatosis. In: Scientific Reports. 2014 ; Vol. 4.
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