DLK-1 as a marker to distinguish unrestricted somatic stem cells and mesenchymal stromal cells in cord blood

Simone Maria Kluth, Anja Buchheiser, Amelie Pia Houben, Stefanie Geyh, Thomas Krenz, Teja Falk Radke, Constanze Wiek, Helmut Hanenberg, Petra Reinecke, Peter Wernet, Gesine Kögler

Research output: Contribution to journalArticle

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Abstract

In addition to hematopoietic stem cells, cord blood (CB) also contains different nonhematopoietic CD45-, CD34- adherent cell populations: cord blood mesenchymal stromal cells (CB MSC) that behave almost like MSC from bone marrow (BM MSC) and unrestricted somatic stem cells (USSC) that differentiate into cells of all 3 germ layers. Distinguishing between these populations is difficult due to overlapping features such as the immunophenotype or the osteogenic and chondrogenic differentiation pathway. Functional differences in the differentiation potential suggest different developmental stages or different cell populations. Here we demonstrate that the expression of genes and the differentiation toward the adipogenic lineage can discriminate between these 2 populations. USSC, including clonal-derived cells lacking adipogenic differentiation, strongly expressed δ-like 1/preadipocyte factor 1 (DLK-1/PREF1) correlating with high proliferative potential, while CB MSC were characterized by a strong differentiation toward adipocytes correlating with a weak or negative DLK-1/PREF1 expression. Constitutive overexpression of DLK-1/PREF1 in CB MSC resulted in a reduced adipogenic differentiation, whereas silencing of DLK-1 in USSC resulted in adipogenic differentiation.

Original languageEnglish
Pages (from-to)1471-1483
Number of pages13
JournalStem Cells and Development
Volume19
Issue number10
DOIs
StatePublished - Oct 1 2010

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Adult Stem Cells
Mesenchymal Stromal Cells
Fetal Blood
Population
Germ Layers
Hematopoietic Stem Cells
Adipocytes
Bone Marrow
Gene Expression

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology

Cite this

Kluth, S. M., Buchheiser, A., Houben, A. P., Geyh, S., Krenz, T., Radke, T. F., ... Kögler, G. (2010). DLK-1 as a marker to distinguish unrestricted somatic stem cells and mesenchymal stromal cells in cord blood. Stem Cells and Development, 19(10), 1471-1483. https://doi.org/10.1089/scd.2010.0070

DLK-1 as a marker to distinguish unrestricted somatic stem cells and mesenchymal stromal cells in cord blood. / Kluth, Simone Maria; Buchheiser, Anja; Houben, Amelie Pia; Geyh, Stefanie; Krenz, Thomas; Radke, Teja Falk; Wiek, Constanze; Hanenberg, Helmut; Reinecke, Petra; Wernet, Peter; Kögler, Gesine.

In: Stem Cells and Development, Vol. 19, No. 10, 01.10.2010, p. 1471-1483.

Research output: Contribution to journalArticle

Kluth, SM, Buchheiser, A, Houben, AP, Geyh, S, Krenz, T, Radke, TF, Wiek, C, Hanenberg, H, Reinecke, P, Wernet, P & Kögler, G 2010, 'DLK-1 as a marker to distinguish unrestricted somatic stem cells and mesenchymal stromal cells in cord blood', Stem Cells and Development, vol. 19, no. 10, pp. 1471-1483. https://doi.org/10.1089/scd.2010.0070
Kluth, Simone Maria ; Buchheiser, Anja ; Houben, Amelie Pia ; Geyh, Stefanie ; Krenz, Thomas ; Radke, Teja Falk ; Wiek, Constanze ; Hanenberg, Helmut ; Reinecke, Petra ; Wernet, Peter ; Kögler, Gesine. / DLK-1 as a marker to distinguish unrestricted somatic stem cells and mesenchymal stromal cells in cord blood. In: Stem Cells and Development. 2010 ; Vol. 19, No. 10. pp. 1471-1483.
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