DNA amplification for DQ typing as an adjunct to serological prenatal HLA typing for the identification of potential donors for umbilical cord blood transplantation

Marilyn S. Pollack, Arleen D. Auerbach, Hal Broxmeyer, Akila Zaafran, Robert L. Griffith, Henry A. Erlich

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

It has recently been demonstrated that umbilical cord blood from genotypically human leukocyte antigen (HLA)-matched donors can provide sufficient numbers of progenitor cells for hematopoietic reconstitution. This technique has been successfully applied in the treatment of two children affected with Fanconi anemia (FA). Fetal cells from the potential sibling donors were first tested to determine that the fetus was not affected with FA. Unaffected fetal cells were then tested for HLA type. Cord blood from compatible donors can be harvested at birth and used immediately or frozen for subsequent use in hematopoietic reconstitution. We now show that fetal cell DNA amplification and hybridization for DQ typing can be an important adjunct procedure to verify serologically determined HLA class II types and/or to establish class II haplotype identity with the affected sibling.

Original languageEnglish
Pages (from-to)45-49
Number of pages5
JournalHuman Immunology
Volume30
Issue number1
DOIs
StatePublished - 1991

Fingerprint

HLA Antigens
Fetal Blood
Fanconi Anemia
Transplantation
Tissue Donors
Siblings
DNA
Hematopoietic Stem Cells
Blood Donors
Haplotypes
Fetus
Parturition
Therapeutics

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

DNA amplification for DQ typing as an adjunct to serological prenatal HLA typing for the identification of potential donors for umbilical cord blood transplantation. / Pollack, Marilyn S.; Auerbach, Arleen D.; Broxmeyer, Hal; Zaafran, Akila; Griffith, Robert L.; Erlich, Henry A.

In: Human Immunology, Vol. 30, No. 1, 1991, p. 45-49.

Research output: Contribution to journalArticle

Pollack, Marilyn S. ; Auerbach, Arleen D. ; Broxmeyer, Hal ; Zaafran, Akila ; Griffith, Robert L. ; Erlich, Henry A. / DNA amplification for DQ typing as an adjunct to serological prenatal HLA typing for the identification of potential donors for umbilical cord blood transplantation. In: Human Immunology. 1991 ; Vol. 30, No. 1. pp. 45-49.
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