DNA-binding properties of the product of the testis-determining gene and a related protein

Nargis Nasrin, Colleen Buggs, Xiang Fu Kong, James Carnazza, Mark Goebl, Maria Alexander-Bridges

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

THE upstream region of the human glyceraldehyde-3-phosphate dehydrogenase gene contains an insulin-response element (IRE-A) responsible for insulin-dependent transcription of the gene (ref. 1). The open reading frame of a rat complementary DNA encoding a protein (IRE-ABP) that binds to this sequence contains an HMG box motif2 that is 67% identical to the mouse candidate gene for the testis-determining factor SRY, and 98% identical to the mouse SAF-like gene, a4 (refs 3, 4). Here we report that IRE-ABP and SRY bind to IRE-A DNA with comparable specificity in a DNase-I footprinting assay. Two females with sex reversal were found to have a single amino-acid substitution in the HMG box domain of SRY at position 3 and 7, respectively5,6. SRY derivatives containing corresponding mutations do not make contact with IRE-A DNA. These results are direct evidence that mouse SRY-like proteins are sequence-specific DNA-binding proteins and identify two amino acids critical to this interaction. Moreover, IRE-A is a candidate SRY-response element.

Original languageEnglish
Pages (from-to)317-320
Number of pages4
JournalNature
Volume354
Issue number6351
StatePublished - Nov 28 1991

Fingerprint

Testis
DNA
Response Elements
Genes
HMG-Box Domains
Sex-Determining Region Y Protein
Proteins
Insulin
Glyceraldehyde-3-Phosphate Dehydrogenases
Deoxyribonuclease I
DNA-Binding Proteins
Amino Acid Substitution
Open Reading Frames
Complementary DNA
Amino Acids
Mutation

ASJC Scopus subject areas

  • General

Cite this

Nasrin, N., Buggs, C., Kong, X. F., Carnazza, J., Goebl, M., & Alexander-Bridges, M. (1991). DNA-binding properties of the product of the testis-determining gene and a related protein. Nature, 354(6351), 317-320.

DNA-binding properties of the product of the testis-determining gene and a related protein. / Nasrin, Nargis; Buggs, Colleen; Kong, Xiang Fu; Carnazza, James; Goebl, Mark; Alexander-Bridges, Maria.

In: Nature, Vol. 354, No. 6351, 28.11.1991, p. 317-320.

Research output: Contribution to journalArticle

Nasrin, N, Buggs, C, Kong, XF, Carnazza, J, Goebl, M & Alexander-Bridges, M 1991, 'DNA-binding properties of the product of the testis-determining gene and a related protein', Nature, vol. 354, no. 6351, pp. 317-320.
Nasrin N, Buggs C, Kong XF, Carnazza J, Goebl M, Alexander-Bridges M. DNA-binding properties of the product of the testis-determining gene and a related protein. Nature. 1991 Nov 28;354(6351):317-320.
Nasrin, Nargis ; Buggs, Colleen ; Kong, Xiang Fu ; Carnazza, James ; Goebl, Mark ; Alexander-Bridges, Maria. / DNA-binding properties of the product of the testis-determining gene and a related protein. In: Nature. 1991 ; Vol. 354, No. 6351. pp. 317-320.
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