DNA-binding properties of the product of the testis-determining gene and a related protein

Nargis Nasrin, Colleen Buggs, Xiang Fu Kong, James Carnazza, Mark Goebl, Maria Alexander-Bridges

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THE upstream region of the human glyceraldehyde-3-phosphate dehydrogenase gene contains an insulin-response element (IRE-A) responsible for insulin-dependent transcription of the gene (ref. 1). The open reading frame of a rat complementary DNA encoding a protein (IRE-ABP) that binds to this sequence contains an HMG box motif2 that is 67% identical to the mouse candidate gene for the testis-determining factor SRY, and 98% identical to the mouse SAF-like gene, a4 (refs 3, 4). Here we report that IRE-ABP and SRY bind to IRE-A DNA with comparable specificity in a DNase-I footprinting assay. Two females with sex reversal were found to have a single amino-acid substitution in the HMG box domain of SRY at position 3 and 7, respectively5,6. SRY derivatives containing corresponding mutations do not make contact with IRE-A DNA. These results are direct evidence that mouse SRY-like proteins are sequence-specific DNA-binding proteins and identify two amino acids critical to this interaction. Moreover, IRE-A is a candidate SRY-response element.

Original languageEnglish (US)
Pages (from-to)317-320
Number of pages4
Issue number6351
StatePublished - Jan 1 1991


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Nasrin, N., Buggs, C., Kong, X. F., Carnazza, J., Goebl, M., & Alexander-Bridges, M. (1991). DNA-binding properties of the product of the testis-determining gene and a related protein. Nature, 354(6351), 317-320. https://doi.org/10.1038/354317a0