DNA content and expression of PCNA and p53 in Hodgkin's disease and Hodgkin's-like B-cell lymphoma

Magdalena Czader, A. Porwit, A. Ost, G. Auer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

DNA ploidy (by image cytometry) and expression of proliferating cell nuclear antigen (PCNA) and p53 tumor suppressor gene product (by immunohistochemistry) were investigated in 15 cases of Hodgkin's disease (HD) and 12 cases of HD-like B-cell lymphoma (HD-like NHL). Reed-Sternberg (RS) cells and their variants were DNA aneuploid in all cases. However, the fraction of hyperoctaploid tumor cells was higher in HD than in HD-like NHL. PCNA expression was high in neoplastic cells (>50%) and variable (5-40%) in reactive lymphocytes in both HD and HD-like NHL. p53 positivity was found in RS cells and their variants in 64% of HD cases, but only in 25% of cases of HD-like NHL. Our results support the suggestion that HD-like B-cell lymphomas should be considered as highly malignant non-Hodgkin's lymphomas rather than Hodgkin's disease.

Original languageEnglish (US)
Pages (from-to)865-873
Number of pages9
JournalAPMIS
Volume102
Issue number11
StatePublished - 1994
Externally publishedYes

Fingerprint

Proliferating Cell Nuclear Antigen
B-Cell Lymphoma
Hodgkin Disease
DNA
Reed-Sternberg Cells
Image Cytometry
Ploidies
Aneuploidy
Tumor Suppressor Genes
Non-Hodgkin's Lymphoma
Immunohistochemistry
Lymphocytes

Keywords

  • B-cell lymphoma
  • DNA aneuploidy
  • DNA image cytometry
  • Hodgkin's disease
  • p53
  • PCNA

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Pathology and Forensic Medicine

Cite this

DNA content and expression of PCNA and p53 in Hodgkin's disease and Hodgkin's-like B-cell lymphoma. / Czader, Magdalena; Porwit, A.; Ost, A.; Auer, G.

In: APMIS, Vol. 102, No. 11, 1994, p. 865-873.

Research output: Contribution to journalArticle

Czader, Magdalena ; Porwit, A. ; Ost, A. ; Auer, G. / DNA content and expression of PCNA and p53 in Hodgkin's disease and Hodgkin's-like B-cell lymphoma. In: APMIS. 1994 ; Vol. 102, No. 11. pp. 865-873.
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