DNA double-strand break repair genes and oxidative damage in brain metastasis of breast cancer

Stephan Woditschka, Lynda Evans, Renata Duchnowska, L. Tiffany Reed, Diane Palmieri, Yongzhen Qian, Sunil Badve, George Sledge, Brunilde Gril, Mirit I. Aladjem, Haiqing Fu, Natasha M. Flores, Yesim Polar, Wojciech Biernat, Ewa Szutowicz-Zielińska, Tomasz Mandat, Tomasz Trojanowski, Waldemar Och, Bogumiła Czartoryska-Arlukowicz, Jacek JassemJames B. Mitchell, Patricia S. Steeg

Research output: Contribution to journalArticle

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Abstract

Background Breast cancer frequently metastasizes to the brain, colonizing a neuro-inflammatory microenvironment. The molecular pathways facilitating this colonization remain poorly understood. Methods Expression profiling of 23 matched sets of human resected brain metastases and primary breast tumors by two-sided paired t test was performed to identify brain metastasis-specific genes. The implicated DNA repair genes BARD1 and RAD51 were modulated in human (MDA-MB-231-BR) and murine (4T1-BR) brain-tropic breast cancer cell lines by lentiviral transduction of cDNA or short hairpin RNA (shRNA) coding sequences. Their functional contribution to brain metastasis development was evaluated in mouse xenograft models (n = 10 mice per group). Results Human brain metastases overexpressed BARD1 and RAD51 compared with either matched primary tumors (1.74-fold, P <. 001; 1.46-fold, P <. 001, respectively) or unlinked systemic metastases (1.49-fold, P =. 01; 1.44-fold, P =. 008, respectively). Overexpression of either gene in MDA-MB-231-BR cells increased brain metastases by threefold to fourfold after intracardiac injections, but not lung metastases upon tail-vein injections. In 4T1-BR cells, shRNA-mediated RAD51 knockdown reduced brain metastases by 2.5-fold without affecting lung metastasis development. In vitro, BARD1- and RAD51-overexpressing cells showed reduced genomic instability but only exhibited growth and colonization phenotypes upon DNA damage induction. Reactive oxygen species were present in tumor cells and elevated in the metastatic neuro-inflammatory microenvironment and could provide an endogenous source of genotoxic stress. Tempol, a brain-permeable oxygen radical scavenger suppressed brain metastasis promotion induced by BARD1 and RAD51 overexpression. Conclusions BARD1 and RAD51 are frequently overexpressed in brain metastases from breast cancer and may constitute a mechanism to overcome reactive oxygen species-mediated genotoxic stress in the metastatic brain.

Original languageEnglish
Article numberdju145
JournalJournal of the National Cancer Institute
Volume106
Issue number7
DOIs
StatePublished - Jul 9 2014

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Double-Stranded DNA Breaks
Breast Neoplasms
Neoplasm Metastasis
Brain
Genes
DNA Damage
Reactive Oxygen Species
Small Interfering RNA
Lung
Injections
Genomic Instability
Heterografts
DNA Repair
Tail
Veins
Neoplasms
Complementary DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Woditschka, S., Evans, L., Duchnowska, R., Reed, L. T., Palmieri, D., Qian, Y., ... Steeg, P. S. (2014). DNA double-strand break repair genes and oxidative damage in brain metastasis of breast cancer. Journal of the National Cancer Institute, 106(7), [dju145]. https://doi.org/10.1093/jnci/dju145

DNA double-strand break repair genes and oxidative damage in brain metastasis of breast cancer. / Woditschka, Stephan; Evans, Lynda; Duchnowska, Renata; Reed, L. Tiffany; Palmieri, Diane; Qian, Yongzhen; Badve, Sunil; Sledge, George; Gril, Brunilde; Aladjem, Mirit I.; Fu, Haiqing; Flores, Natasha M.; Polar, Yesim; Biernat, Wojciech; Szutowicz-Zielińska, Ewa; Mandat, Tomasz; Trojanowski, Tomasz; Och, Waldemar; Czartoryska-Arlukowicz, Bogumiła; Jassem, Jacek; Mitchell, James B.; Steeg, Patricia S.

In: Journal of the National Cancer Institute, Vol. 106, No. 7, dju145, 09.07.2014.

Research output: Contribution to journalArticle

Woditschka, S, Evans, L, Duchnowska, R, Reed, LT, Palmieri, D, Qian, Y, Badve, S, Sledge, G, Gril, B, Aladjem, MI, Fu, H, Flores, NM, Polar, Y, Biernat, W, Szutowicz-Zielińska, E, Mandat, T, Trojanowski, T, Och, W, Czartoryska-Arlukowicz, B, Jassem, J, Mitchell, JB & Steeg, PS 2014, 'DNA double-strand break repair genes and oxidative damage in brain metastasis of breast cancer', Journal of the National Cancer Institute, vol. 106, no. 7, dju145. https://doi.org/10.1093/jnci/dju145
Woditschka, Stephan ; Evans, Lynda ; Duchnowska, Renata ; Reed, L. Tiffany ; Palmieri, Diane ; Qian, Yongzhen ; Badve, Sunil ; Sledge, George ; Gril, Brunilde ; Aladjem, Mirit I. ; Fu, Haiqing ; Flores, Natasha M. ; Polar, Yesim ; Biernat, Wojciech ; Szutowicz-Zielińska, Ewa ; Mandat, Tomasz ; Trojanowski, Tomasz ; Och, Waldemar ; Czartoryska-Arlukowicz, Bogumiła ; Jassem, Jacek ; Mitchell, James B. ; Steeg, Patricia S. / DNA double-strand break repair genes and oxidative damage in brain metastasis of breast cancer. In: Journal of the National Cancer Institute. 2014 ; Vol. 106, No. 7.
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abstract = "Background Breast cancer frequently metastasizes to the brain, colonizing a neuro-inflammatory microenvironment. The molecular pathways facilitating this colonization remain poorly understood. Methods Expression profiling of 23 matched sets of human resected brain metastases and primary breast tumors by two-sided paired t test was performed to identify brain metastasis-specific genes. The implicated DNA repair genes BARD1 and RAD51 were modulated in human (MDA-MB-231-BR) and murine (4T1-BR) brain-tropic breast cancer cell lines by lentiviral transduction of cDNA or short hairpin RNA (shRNA) coding sequences. Their functional contribution to brain metastasis development was evaluated in mouse xenograft models (n = 10 mice per group). Results Human brain metastases overexpressed BARD1 and RAD51 compared with either matched primary tumors (1.74-fold, P <. 001; 1.46-fold, P <. 001, respectively) or unlinked systemic metastases (1.49-fold, P =. 01; 1.44-fold, P =. 008, respectively). Overexpression of either gene in MDA-MB-231-BR cells increased brain metastases by threefold to fourfold after intracardiac injections, but not lung metastases upon tail-vein injections. In 4T1-BR cells, shRNA-mediated RAD51 knockdown reduced brain metastases by 2.5-fold without affecting lung metastasis development. In vitro, BARD1- and RAD51-overexpressing cells showed reduced genomic instability but only exhibited growth and colonization phenotypes upon DNA damage induction. Reactive oxygen species were present in tumor cells and elevated in the metastatic neuro-inflammatory microenvironment and could provide an endogenous source of genotoxic stress. Tempol, a brain-permeable oxygen radical scavenger suppressed brain metastasis promotion induced by BARD1 and RAD51 overexpression. Conclusions BARD1 and RAD51 are frequently overexpressed in brain metastases from breast cancer and may constitute a mechanism to overcome reactive oxygen species-mediated genotoxic stress in the metastatic brain.",
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T1 - DNA double-strand break repair genes and oxidative damage in brain metastasis of breast cancer

AU - Woditschka, Stephan

AU - Evans, Lynda

AU - Duchnowska, Renata

AU - Reed, L. Tiffany

AU - Palmieri, Diane

AU - Qian, Yongzhen

AU - Badve, Sunil

AU - Sledge, George

AU - Gril, Brunilde

AU - Aladjem, Mirit I.

AU - Fu, Haiqing

AU - Flores, Natasha M.

AU - Polar, Yesim

AU - Biernat, Wojciech

AU - Szutowicz-Zielińska, Ewa

AU - Mandat, Tomasz

AU - Trojanowski, Tomasz

AU - Och, Waldemar

AU - Czartoryska-Arlukowicz, Bogumiła

AU - Jassem, Jacek

AU - Mitchell, James B.

AU - Steeg, Patricia S.

PY - 2014/7/9

Y1 - 2014/7/9

N2 - Background Breast cancer frequently metastasizes to the brain, colonizing a neuro-inflammatory microenvironment. The molecular pathways facilitating this colonization remain poorly understood. Methods Expression profiling of 23 matched sets of human resected brain metastases and primary breast tumors by two-sided paired t test was performed to identify brain metastasis-specific genes. The implicated DNA repair genes BARD1 and RAD51 were modulated in human (MDA-MB-231-BR) and murine (4T1-BR) brain-tropic breast cancer cell lines by lentiviral transduction of cDNA or short hairpin RNA (shRNA) coding sequences. Their functional contribution to brain metastasis development was evaluated in mouse xenograft models (n = 10 mice per group). Results Human brain metastases overexpressed BARD1 and RAD51 compared with either matched primary tumors (1.74-fold, P <. 001; 1.46-fold, P <. 001, respectively) or unlinked systemic metastases (1.49-fold, P =. 01; 1.44-fold, P =. 008, respectively). Overexpression of either gene in MDA-MB-231-BR cells increased brain metastases by threefold to fourfold after intracardiac injections, but not lung metastases upon tail-vein injections. In 4T1-BR cells, shRNA-mediated RAD51 knockdown reduced brain metastases by 2.5-fold without affecting lung metastasis development. In vitro, BARD1- and RAD51-overexpressing cells showed reduced genomic instability but only exhibited growth and colonization phenotypes upon DNA damage induction. Reactive oxygen species were present in tumor cells and elevated in the metastatic neuro-inflammatory microenvironment and could provide an endogenous source of genotoxic stress. Tempol, a brain-permeable oxygen radical scavenger suppressed brain metastasis promotion induced by BARD1 and RAD51 overexpression. Conclusions BARD1 and RAD51 are frequently overexpressed in brain metastases from breast cancer and may constitute a mechanism to overcome reactive oxygen species-mediated genotoxic stress in the metastatic brain.

AB - Background Breast cancer frequently metastasizes to the brain, colonizing a neuro-inflammatory microenvironment. The molecular pathways facilitating this colonization remain poorly understood. Methods Expression profiling of 23 matched sets of human resected brain metastases and primary breast tumors by two-sided paired t test was performed to identify brain metastasis-specific genes. The implicated DNA repair genes BARD1 and RAD51 were modulated in human (MDA-MB-231-BR) and murine (4T1-BR) brain-tropic breast cancer cell lines by lentiviral transduction of cDNA or short hairpin RNA (shRNA) coding sequences. Their functional contribution to brain metastasis development was evaluated in mouse xenograft models (n = 10 mice per group). Results Human brain metastases overexpressed BARD1 and RAD51 compared with either matched primary tumors (1.74-fold, P <. 001; 1.46-fold, P <. 001, respectively) or unlinked systemic metastases (1.49-fold, P =. 01; 1.44-fold, P =. 008, respectively). Overexpression of either gene in MDA-MB-231-BR cells increased brain metastases by threefold to fourfold after intracardiac injections, but not lung metastases upon tail-vein injections. In 4T1-BR cells, shRNA-mediated RAD51 knockdown reduced brain metastases by 2.5-fold without affecting lung metastasis development. In vitro, BARD1- and RAD51-overexpressing cells showed reduced genomic instability but only exhibited growth and colonization phenotypes upon DNA damage induction. Reactive oxygen species were present in tumor cells and elevated in the metastatic neuro-inflammatory microenvironment and could provide an endogenous source of genotoxic stress. Tempol, a brain-permeable oxygen radical scavenger suppressed brain metastasis promotion induced by BARD1 and RAD51 overexpression. Conclusions BARD1 and RAD51 are frequently overexpressed in brain metastases from breast cancer and may constitute a mechanism to overcome reactive oxygen species-mediated genotoxic stress in the metastatic brain.

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