DNA image analysis in childhood acute lymphoblastic leukemia

Magdalena Czader, Anna Porwit, Stefan Söderhäll, Elisabeth Blennow, Susanne Widell, Peter Reizenstein, Åke Öst, Gert Auer

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

DNA index (DI) and percentages of cells in S and G2/M phase were determined in Feulgen stained nuclei of blasts from 31 cases of childhood ALL at diagnosis. In 6 cases the results of DNA analysis and cytogenetics were concordant showing hyperdiploidy. Two other cases with normal karyotype were revealed as DNA aneuploid with image analysis. Cases with cytogenetic abnormalities like translocation, deletion or presence of single or double supernumerary chromosomes had DI within normal ranges. Nine ALL cases (29% were found to be DNA aneuploid-8 hyperdiploid and 1 hypodiploid. The percentages of cells in S and G2/M phase for blasts from bone marrow (mean 17.6% were significantly higher than those estimated in the peripheral blood (mean 1.57% We conclude that analysis by image cytometry can detect aneuploid DNA content even in cases, which showed a normal karyotype and provides new information concerning the biological aspects of leukemic blasts.

Original languageEnglish (US)
Pages (from-to)229-235
Number of pages7
JournalLeukemia and Lymphoma
Volume9
Issue number3
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Keywords

  • Childhood ALL
  • DNA aneuploidy
  • Image cytometry

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Czader, M., Porwit, A., Söderhäll, S., Blennow, E., Widell, S., Reizenstein, P., Öst, Å., & Auer, G. (1993). DNA image analysis in childhood acute lymphoblastic leukemia. Leukemia and Lymphoma, 9(3), 229-235. https://doi.org/10.3109/10428199309147375