DNA image cytometry and the expression of proliferative markers (proliferating cell nuclear antigen and Ki67) in non-Hodgkin's lymphomas

Magdalena Czader, A. Porwit, E. Tani, A. Ost, J. Mazur, G. Auer

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have analyzed DNA content and proliferative activity in morphologically defined cell subpopulations of 74 non-Hodgkin's lymphomas (NHL) and 29 reactive lymph nodes using DNA image cytometry and antibodies to proliferative markers (proliferating cell nuclear antigen (PCNA) and Ki67). Thirteen (18.6%) of 70 NHL cases were aneuploid. The follicular center cell-derived lymphomas with DNA aneuploidy had DNA indices (DI) predominantly in the tetraploid region, whereas aneuploid high-grade (HG) NHL presented DNA histograms with multiple aneuploid stemlines. In aneuploid centrocytic-centroblastic (CB/CC) NHLs, DNA aneuploidy was found exclusively in centroblasts, whereas centrocytes in these cases were diploid. Percentages of cells in S and G2/M phase in chronic lymphocytic leukemia (CLL), immunocytoma (IC), centrocytic NHL (CC), and centrocytes from CB/CC were low (<5%), whereas the respective values for centroblasts in CB/CC and in malignant cells of HG NHL were similar to those of large lymphoid cells in the reactive lymph nodes (mean, 39.5%, 36.6%, and 53.5%, respectively). The mean percentage of PCNA positive cells in CLL, IC, and CC was 4.9%. In the follicles of CB/CC NHLs there was, on average, 56.9% of PCNA positive centroblasts and 8.1% of PCNA positive centrocytes. In HG NHL, the mean percentage of PCNA positive lymphoma cells was 27.9%. A positive correlation was found between percentages of cells in S and G2/M phase and cells positive for PCNA (P <0.001). There was also a significant correlation between percentages of Ki67 (mean, 19.2%) and PCNA positive cells (mean, 17.7%) (P <0.01). The histogram of frequency distribution for the S and G2/M fractions and for fractions positive for PCNA was asymmetric, with the cut-off value at approximately 30%. We conclude that DNA image cytometry detects aneuploidy even in small subpopulations of lymphoma cells and that anti-PCNA antibody can be used to determine proliferative fraction in paraffin-embedded material from NHL.

Original languageEnglish (US)
Pages (from-to)51-58
Number of pages8
JournalModern Pathology
Volume8
Issue number1
StatePublished - 1995
Externally publishedYes

Fingerprint

Image Cytometry
Ki-67 Antigen
Proliferating Cell Nuclear Antigen
Non-Hodgkin's Lymphoma
Aneuploidy
DNA
B-Cell Chronic Lymphocytic Leukemia
G2 Phase
Cell Division
Lymphoma
Lymph Nodes
Tetraploidy
Antibodies
Diploidy
Paraffin

Keywords

  • DNA ploidy
  • Ki67
  • non-Hogdkin's lymphoma
  • PCNA
  • proliferation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

DNA image cytometry and the expression of proliferative markers (proliferating cell nuclear antigen and Ki67) in non-Hodgkin's lymphomas. / Czader, Magdalena; Porwit, A.; Tani, E.; Ost, A.; Mazur, J.; Auer, G.

In: Modern Pathology, Vol. 8, No. 1, 1995, p. 51-58.

Research output: Contribution to journalArticle

@article{8fbb1b0176a34d2e94bf35f90bf1974b,
title = "DNA image cytometry and the expression of proliferative markers (proliferating cell nuclear antigen and Ki67) in non-Hodgkin's lymphomas",
abstract = "We have analyzed DNA content and proliferative activity in morphologically defined cell subpopulations of 74 non-Hodgkin's lymphomas (NHL) and 29 reactive lymph nodes using DNA image cytometry and antibodies to proliferative markers (proliferating cell nuclear antigen (PCNA) and Ki67). Thirteen (18.6{\%}) of 70 NHL cases were aneuploid. The follicular center cell-derived lymphomas with DNA aneuploidy had DNA indices (DI) predominantly in the tetraploid region, whereas aneuploid high-grade (HG) NHL presented DNA histograms with multiple aneuploid stemlines. In aneuploid centrocytic-centroblastic (CB/CC) NHLs, DNA aneuploidy was found exclusively in centroblasts, whereas centrocytes in these cases were diploid. Percentages of cells in S and G2/M phase in chronic lymphocytic leukemia (CLL), immunocytoma (IC), centrocytic NHL (CC), and centrocytes from CB/CC were low (<5{\%}), whereas the respective values for centroblasts in CB/CC and in malignant cells of HG NHL were similar to those of large lymphoid cells in the reactive lymph nodes (mean, 39.5{\%}, 36.6{\%}, and 53.5{\%}, respectively). The mean percentage of PCNA positive cells in CLL, IC, and CC was 4.9{\%}. In the follicles of CB/CC NHLs there was, on average, 56.9{\%} of PCNA positive centroblasts and 8.1{\%} of PCNA positive centrocytes. In HG NHL, the mean percentage of PCNA positive lymphoma cells was 27.9{\%}. A positive correlation was found between percentages of cells in S and G2/M phase and cells positive for PCNA (P <0.001). There was also a significant correlation between percentages of Ki67 (mean, 19.2{\%}) and PCNA positive cells (mean, 17.7{\%}) (P <0.01). The histogram of frequency distribution for the S and G2/M fractions and for fractions positive for PCNA was asymmetric, with the cut-off value at approximately 30{\%}. We conclude that DNA image cytometry detects aneuploidy even in small subpopulations of lymphoma cells and that anti-PCNA antibody can be used to determine proliferative fraction in paraffin-embedded material from NHL.",
keywords = "DNA ploidy, Ki67, non-Hogdkin's lymphoma, PCNA, proliferation",
author = "Magdalena Czader and A. Porwit and E. Tani and A. Ost and J. Mazur and G. Auer",
year = "1995",
language = "English (US)",
volume = "8",
pages = "51--58",
journal = "Modern Pathology",
issn = "0893-3952",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - DNA image cytometry and the expression of proliferative markers (proliferating cell nuclear antigen and Ki67) in non-Hodgkin's lymphomas

AU - Czader, Magdalena

AU - Porwit, A.

AU - Tani, E.

AU - Ost, A.

AU - Mazur, J.

AU - Auer, G.

PY - 1995

Y1 - 1995

N2 - We have analyzed DNA content and proliferative activity in morphologically defined cell subpopulations of 74 non-Hodgkin's lymphomas (NHL) and 29 reactive lymph nodes using DNA image cytometry and antibodies to proliferative markers (proliferating cell nuclear antigen (PCNA) and Ki67). Thirteen (18.6%) of 70 NHL cases were aneuploid. The follicular center cell-derived lymphomas with DNA aneuploidy had DNA indices (DI) predominantly in the tetraploid region, whereas aneuploid high-grade (HG) NHL presented DNA histograms with multiple aneuploid stemlines. In aneuploid centrocytic-centroblastic (CB/CC) NHLs, DNA aneuploidy was found exclusively in centroblasts, whereas centrocytes in these cases were diploid. Percentages of cells in S and G2/M phase in chronic lymphocytic leukemia (CLL), immunocytoma (IC), centrocytic NHL (CC), and centrocytes from CB/CC were low (<5%), whereas the respective values for centroblasts in CB/CC and in malignant cells of HG NHL were similar to those of large lymphoid cells in the reactive lymph nodes (mean, 39.5%, 36.6%, and 53.5%, respectively). The mean percentage of PCNA positive cells in CLL, IC, and CC was 4.9%. In the follicles of CB/CC NHLs there was, on average, 56.9% of PCNA positive centroblasts and 8.1% of PCNA positive centrocytes. In HG NHL, the mean percentage of PCNA positive lymphoma cells was 27.9%. A positive correlation was found between percentages of cells in S and G2/M phase and cells positive for PCNA (P <0.001). There was also a significant correlation between percentages of Ki67 (mean, 19.2%) and PCNA positive cells (mean, 17.7%) (P <0.01). The histogram of frequency distribution for the S and G2/M fractions and for fractions positive for PCNA was asymmetric, with the cut-off value at approximately 30%. We conclude that DNA image cytometry detects aneuploidy even in small subpopulations of lymphoma cells and that anti-PCNA antibody can be used to determine proliferative fraction in paraffin-embedded material from NHL.

AB - We have analyzed DNA content and proliferative activity in morphologically defined cell subpopulations of 74 non-Hodgkin's lymphomas (NHL) and 29 reactive lymph nodes using DNA image cytometry and antibodies to proliferative markers (proliferating cell nuclear antigen (PCNA) and Ki67). Thirteen (18.6%) of 70 NHL cases were aneuploid. The follicular center cell-derived lymphomas with DNA aneuploidy had DNA indices (DI) predominantly in the tetraploid region, whereas aneuploid high-grade (HG) NHL presented DNA histograms with multiple aneuploid stemlines. In aneuploid centrocytic-centroblastic (CB/CC) NHLs, DNA aneuploidy was found exclusively in centroblasts, whereas centrocytes in these cases were diploid. Percentages of cells in S and G2/M phase in chronic lymphocytic leukemia (CLL), immunocytoma (IC), centrocytic NHL (CC), and centrocytes from CB/CC were low (<5%), whereas the respective values for centroblasts in CB/CC and in malignant cells of HG NHL were similar to those of large lymphoid cells in the reactive lymph nodes (mean, 39.5%, 36.6%, and 53.5%, respectively). The mean percentage of PCNA positive cells in CLL, IC, and CC was 4.9%. In the follicles of CB/CC NHLs there was, on average, 56.9% of PCNA positive centroblasts and 8.1% of PCNA positive centrocytes. In HG NHL, the mean percentage of PCNA positive lymphoma cells was 27.9%. A positive correlation was found between percentages of cells in S and G2/M phase and cells positive for PCNA (P <0.001). There was also a significant correlation between percentages of Ki67 (mean, 19.2%) and PCNA positive cells (mean, 17.7%) (P <0.01). The histogram of frequency distribution for the S and G2/M fractions and for fractions positive for PCNA was asymmetric, with the cut-off value at approximately 30%. We conclude that DNA image cytometry detects aneuploidy even in small subpopulations of lymphoma cells and that anti-PCNA antibody can be used to determine proliferative fraction in paraffin-embedded material from NHL.

KW - DNA ploidy

KW - Ki67

KW - non-Hogdkin's lymphoma

KW - PCNA

KW - proliferation

UR - http://www.scopus.com/inward/record.url?scp=0028816688&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028816688&partnerID=8YFLogxK

M3 - Article

C2 - 7731942

AN - SCOPUS:0028816688

VL - 8

SP - 51

EP - 58

JO - Modern Pathology

JF - Modern Pathology

SN - 0893-3952

IS - 1

ER -