DNA repair-redox enzyme apurinic endonuclease in cervical cancer: evaluation of redox control of HIF-1alpha and prognostic significance.

M. Schindl, G. Oberhuber, E. G. Pichlbauer, A. Obermair, P. Birner, Mark Kelley

Research output: Contribution to journalArticle

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Abstract

The multifunctional apurinic/apyrimidinic endonuclease (Ape1/ref-1) plays a key role in the human DNA base excision repair pathway. Ape1/ref-1 has also been shown to be involved in the redox control of transactivation activities of hypoxia-inducible factor (HIF)-1alpha. The aim of our study was to investigate the expression of these proteins in early stage invasive cervical cancer. Expression of Ape1/ref-1 and HIF-1alpha was detected immunohistochemically in 88 samples of cervical cancer stage pT1b. The levels of the proteins were compared and the prognostic influence of Ape1/ref-1 expression was investigated. Strong nuclear expression of Ape1/ref-1 was observed in 9 cases (10.2%), moderate in 22 cases (25%), weak in 17 cases (19.3%), and absent in 40 cases (45.5%). Furthermore, no correlation between Ape1/ref-1 and HIF-1alpha expression was observed (p=0.864). We also found no relationship of Ape1/ref-1 expression and survival (p>0.05, log-rank test). From these studies, we have concluded that in cervical cancer there is no correlation between the upstream redox regulatory protein of HIF-1, i.e., Ape1/ref-1, and HIF-1alpha expression. However, these studies do not address any functional relationship between the two proteins.

Original languageEnglish (US)
Pages (from-to)799-802
Number of pages4
JournalInternational Journal of Oncology
Volume19
Issue number4
StatePublished - Oct 2001
Externally publishedYes

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DNA-(Apurinic or Apyrimidinic Site) Lyase
DNA Repair Enzymes
Hypoxia-Inducible Factor 1
Uterine Cervical Neoplasms
Oxidation-Reduction
Proteins
DNA Repair
Transcriptional Activation
Survival
Hypoxia
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

DNA repair-redox enzyme apurinic endonuclease in cervical cancer : evaluation of redox control of HIF-1alpha and prognostic significance. / Schindl, M.; Oberhuber, G.; Pichlbauer, E. G.; Obermair, A.; Birner, P.; Kelley, Mark.

In: International Journal of Oncology, Vol. 19, No. 4, 10.2001, p. 799-802.

Research output: Contribution to journalArticle

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abstract = "The multifunctional apurinic/apyrimidinic endonuclease (Ape1/ref-1) plays a key role in the human DNA base excision repair pathway. Ape1/ref-1 has also been shown to be involved in the redox control of transactivation activities of hypoxia-inducible factor (HIF)-1alpha. The aim of our study was to investigate the expression of these proteins in early stage invasive cervical cancer. Expression of Ape1/ref-1 and HIF-1alpha was detected immunohistochemically in 88 samples of cervical cancer stage pT1b. The levels of the proteins were compared and the prognostic influence of Ape1/ref-1 expression was investigated. Strong nuclear expression of Ape1/ref-1 was observed in 9 cases (10.2{\%}), moderate in 22 cases (25{\%}), weak in 17 cases (19.3{\%}), and absent in 40 cases (45.5{\%}). Furthermore, no correlation between Ape1/ref-1 and HIF-1alpha expression was observed (p=0.864). We also found no relationship of Ape1/ref-1 expression and survival (p>0.05, log-rank test). From these studies, we have concluded that in cervical cancer there is no correlation between the upstream redox regulatory protein of HIF-1, i.e., Ape1/ref-1, and HIF-1alpha expression. However, these studies do not address any functional relationship between the two proteins.",
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