DNA sequence analysis of prm mutations of coliphage lambda

Elliot D. Rosen, James L. Hartley, Karen Matz, Brian P. Nichols, Kathleen M. Young, John E. Donelson, Gary N. Gussin

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Nucleotide sequence changes associated with mutation of the prm promoter of bacteriophage λ have been determined. Prm mutations have been assigned to two classes. Class I mutations appear to affect the interaction of RNA polymerase with prm ;six class I mutations affect four sites, located 14, 33, 38, and 39 bp preceding the prm transcription startpoint. Class II mutations appear to owe their Prm phenotype to a change in oR, which could prevent activation of prm by repressor. All three class II mutations are in oR 1.

Original languageEnglish (US)
Pages (from-to)197-205
Number of pages9
JournalGene
Volume11
Issue number3-4
DOIs
StatePublished - Jan 1 1980
Externally publishedYes

Keywords

  • Bacteriophage λ
  • Prm
  • base pairs
  • bp
  • mutations in p
  • phenotype characteristics of prm mutations
  • prm
  • prm
  • promoter for repressor mRNA
  • promotor for repressor maintenance
  • regulation of transcription

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Rosen, E. D., Hartley, J. L., Matz, K., Nichols, B. P., Young, K. M., Donelson, J. E., & Gussin, G. N. (1980). DNA sequence analysis of prm mutations of coliphage lambda. Gene, 11(3-4), 197-205. https://doi.org/10.1016/0378-1119(80)90060-8