DNA sequence evidence for polymorphic forms of human serum amyloid A (SAA)

Barbara Kluve-Beckerman, George L. Long, Merrill Benson

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Serum amyloid A (SAA) is an acute-phase reactant and precursor to amyloid A protein, the major constituent of the fibril deposits of reactive amyloidosis. The factors determining whether the 104-amino acid SAA molecule is converted into the 76-amino acid amyloid A protein and deposited as fibrils are not known. As an initial step toward investigating the possibility that a particular primary structure of SAA is involved in amyloid formation, we have cloned and determined the nucleotide sequence of human SAA-specific cDNAs. The first clone, selected using an oligonucleotide probe, was shown to encode the signal peptide and amino-terminal region of SAA. The cDNA of this clone served as probe in the selection of two distinct, full-length SAA cDNAs, initially differentiated by the presence (pSAA21) or absence (pSAA82) of a PstI site in the coding sequence. The complete nucleotide sequence of pSAA82 cDNA was determined. Since there appear to be multiple human SAA alleles, it is conceivable that their differential expression is important to amyloid formation.

Original languageEnglish
Pages (from-to)795-803
Number of pages9
JournalBiochemical Genetics
Volume24
Issue number11-12
DOIs
StatePublished - Dec 1986

Fingerprint

Serum Amyloid A Protein
DNA sequences
amyloid
serum
nucleotide sequences
DNA
Complementary DNA
clone
amino acid
Amyloid
probe
Nucleotides
Clone Cells
protein
Amino Acids
peptide
Oligonucleotide Probes
Acute-Phase Proteins
clones
Amyloidosis

Keywords

  • amyloidosis
  • cDNA clone
  • polymorphism
  • serum amyloid A

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

DNA sequence evidence for polymorphic forms of human serum amyloid A (SAA). / Kluve-Beckerman, Barbara; Long, George L.; Benson, Merrill.

In: Biochemical Genetics, Vol. 24, No. 11-12, 12.1986, p. 795-803.

Research output: Contribution to journalArticle

Kluve-Beckerman, Barbara ; Long, George L. ; Benson, Merrill. / DNA sequence evidence for polymorphic forms of human serum amyloid A (SAA). In: Biochemical Genetics. 1986 ; Vol. 24, No. 11-12. pp. 795-803.
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