DNA specificity determinants associate with distinct transcription factor functions

Peter Hollenhorst, Katherine J. Chandler, Rachel L. Poulsen, W. Evan Johnson, Nancy A. Speck, Barbara J. Graves

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

To elucidate how genomic sequences build transcriptional control networks, we need to understand the connection between DNA sequence and transcription factor binding and function. Binding predictions based solely on consensus predictions are limited, because a single factor can use degenerate sequence motifs and because related transcription factors often prefer identical sequences. The ETS family transcription factor, ETS1, exemplifies these challenges. Unexpected, redundant occupancy of ETS1 and other ETS proteins is observed at promoters of housekeeping genes in T cells due to common sequence preferences and the presence of strong consensus motifs. However, ETS1 exhibits a specific function in T cell activation; thus, unique transcriptional targets are predicted. To uncover the sequence motifs that mediate specific functions of ETS1, a genome-wide approach, chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq), identified both promoter and enhancer binding events in Jurkat T cells. A comparison with DNase I sensitivity both validated the dataset and also improved accuracy. Redundant occupancy of ETS1 with the ETS protein GABPA occurred primarily in promoters of housekeeping genes, whereas ETS1 specific occupancy occurred in the enhancers of T cell-specific genes. Two routes to ETS1 specificity were identified: an intrinsic preference of ETS1 for a variant of the ETS family consensus sequence and the presence of a composite sequence that can support cooperative binding with a RUNX transcription factor. Genome-wide occupancy of RUNX factors corroborated the importance of this partnership. Furthermore, genome-wide occupancy of co-activator CBP indicated tight co-localization with ETS1 at specific enhancers, but not redundant promoters. The distinct sequences associated with redundant versus specific ETS1 occupancy were predictive of promoter or enhancer location and the ontology of nearby genes. These findings demonstrate that diversity of DNA binding motifs may enable variable transcription factor function at different genomic sites.

Original languageEnglish (US)
Article numbere1000778
JournalPLoS Genetics
Volume5
Issue number12
DOIs
StatePublished - Dec 2009
Externally publishedYes

Fingerprint

Transcription Factors
transcription factors
promoter regions
DNA
T-lymphocytes
genome
gene
T-Lymphocytes
Essential Genes
Genome
genomics
protein
deoxyribonuclease I
prediction
Nucleotide Motifs
Gene Ontology
Jurkat Cells
DNA-binding domains
consensus sequence
Gene Regulatory Networks

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Hollenhorst, P., Chandler, K. J., Poulsen, R. L., Johnson, W. E., Speck, N. A., & Graves, B. J. (2009). DNA specificity determinants associate with distinct transcription factor functions. PLoS Genetics, 5(12), [e1000778]. https://doi.org/10.1371/journal.pgen.1000778

DNA specificity determinants associate with distinct transcription factor functions. / Hollenhorst, Peter; Chandler, Katherine J.; Poulsen, Rachel L.; Johnson, W. Evan; Speck, Nancy A.; Graves, Barbara J.

In: PLoS Genetics, Vol. 5, No. 12, e1000778, 12.2009.

Research output: Contribution to journalArticle

Hollenhorst, P, Chandler, KJ, Poulsen, RL, Johnson, WE, Speck, NA & Graves, BJ 2009, 'DNA specificity determinants associate with distinct transcription factor functions', PLoS Genetics, vol. 5, no. 12, e1000778. https://doi.org/10.1371/journal.pgen.1000778
Hollenhorst, Peter ; Chandler, Katherine J. ; Poulsen, Rachel L. ; Johnson, W. Evan ; Speck, Nancy A. ; Graves, Barbara J. / DNA specificity determinants associate with distinct transcription factor functions. In: PLoS Genetics. 2009 ; Vol. 5, No. 12.
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