DNA synthesis by partially purified replicating simian virus 40 chromosomes

Howard J. Edenberg, M. Anwar Waqar, Joel A. Huberman

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

We have partially purified replicating simian virus 40 (SV40) chromosomes in a form which allows continued DNA synthesis in vitro. We first prepare a soluble DNA-synthesizing system from SV40-infected monkey cells and then sediment the components through a neutral sucrose gradient of extremely low ionic strength. Replicating SV40 chromosomes isolated from such gradients are capable of continuing DNA synthesis in vitro in the same manner as two crude subnuclear systems we have previously described (4). This indicates that the enzymes and other proteins required for in vitro DNA synthesis are bound to the replicating chromosomes.

Original languageEnglish (US)
Pages (from-to)3083-3096
Number of pages14
JournalNucleic acids research
Volume4
Issue number9
DOIs
StatePublished - Sep 1977

ASJC Scopus subject areas

  • Genetics

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