DNA synthesis by partially purified replicating simian virus 40 chromosomes

Howard Edenberg, M. Anwar Waqar, Joel A. Huberman

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have partially purified replicating simian virus 40 (SV40) chromosomes in a form which allows continued DNA synthesis in vitro. We first prepare a soluble DNA-synthesizing system from SV40-infected monkey cells and then sediment the components through a neutral sucrose gradient of extremely low ionic strength. Replicating SV40 chromosomes isolated from such gradients are capable of continuing DNA synthesis in vitro in the same manner as two crude subnuclear systems we have previously described (4). This indicates that the enzymes and other proteins required for in vitro DNA synthesis are bound to the replicating chromosomes.

Original languageEnglish (US)
Pages (from-to)3083-3096
Number of pages14
JournalNucleic Acids Research
Volume4
Issue number9
DOIs
StatePublished - Sep 1977
Externally publishedYes

Fingerprint

Simian virus 40
Chromosomes
Viruses
Virus
Chromosome
DNA
Synthesis
Gradient
Sucrose
Sediment
Sugar (sucrose)
Enzymes
Ionic strength
Osmolar Concentration
Haplorhini
Protein
Sediments
Cell
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

DNA synthesis by partially purified replicating simian virus 40 chromosomes. / Edenberg, Howard; Waqar, M. Anwar; Huberman, Joel A.

In: Nucleic Acids Research, Vol. 4, No. 9, 09.1977, p. 3083-3096.

Research output: Contribution to journalArticle

Edenberg, Howard ; Waqar, M. Anwar ; Huberman, Joel A. / DNA synthesis by partially purified replicating simian virus 40 chromosomes. In: Nucleic Acids Research. 1977 ; Vol. 4, No. 9. pp. 3083-3096.
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