DNA vaccine against NgR promotes functional recovery after spinal cord injury in adult rats

Panpan Yu, Lidong Huang, Jian Zou, Huiqing Zhu, Xiaofei Wang, Zhihua Yu, Xiao-Ming Xu, Pei Hua Lu

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

NgR is a common receptor for three myelin-associated inhibitors and mediates their inhibitory activities on neurite outgrowth. In the present study, we investigated whether a DNA vaccine targeting NgR could play a beneficial role in improving recovery from spinal cord injury (SCI). We demonstrated that a DNA vaccine against NgR was successfully constructed and expressed efficiently in vitro and in vivo. After immunization with anti-NgR DNA vaccine, a low level of antibody response and a T cell-mediated immune response were induced in the vaccinated rats. And the antisera taken from the anti-NgR DNA vaccinated rats could partly reverse the inhibition of MAG on neurite outgrowth. When the rats were subjected to a contusive SCI, the vaccinated rats showed much better functional recovery than the controls. In those vaccinated rats that induced a T cell response and generated antibodies against NgR, functional improvements were even better. Histological assessments by three-dimensional reconstruction further demonstrated that the total lesion volume in the vaccinated rats was reduced by 30.8% compared to the controls. These results collectively suggest that DNA vaccine against NgR can significantly improve functional recovery in rats that received contusive SCI and that the vaccination approach may provide a promising strategy for promoting SCI repair.

Original languageEnglish (US)
Pages (from-to)66-76
Number of pages11
JournalBrain Research
Volume1147
Issue number1
DOIs
StatePublished - May 25 2007
Externally publishedYes

Fingerprint

DNA Vaccines
Spinal Cord Injuries
Antibody Formation
Spinal Cord Regeneration
T-Lymphocytes
Myelin Sheath
Immune Sera
Immunization
Vaccination
DNA

Keywords

  • DNA vaccine
  • Functional recovery
  • Myelin-associated inhibitor
  • NgR
  • Spinal cord injury

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

DNA vaccine against NgR promotes functional recovery after spinal cord injury in adult rats. / Yu, Panpan; Huang, Lidong; Zou, Jian; Zhu, Huiqing; Wang, Xiaofei; Yu, Zhihua; Xu, Xiao-Ming; Lu, Pei Hua.

In: Brain Research, Vol. 1147, No. 1, 25.05.2007, p. 66-76.

Research output: Contribution to journalArticle

Yu, Panpan ; Huang, Lidong ; Zou, Jian ; Zhu, Huiqing ; Wang, Xiaofei ; Yu, Zhihua ; Xu, Xiao-Ming ; Lu, Pei Hua. / DNA vaccine against NgR promotes functional recovery after spinal cord injury in adult rats. In: Brain Research. 2007 ; Vol. 1147, No. 1. pp. 66-76.
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