Does endogenous testosterone mediate the lower preconditioning threshold in males?

Nicholas T. Nelson, Adam J. Mahomed, Jeffrey M. Pitcher, Ben M. Tsai, Meijing Wang, Yousuf Mahomed, John Brown, Paul Crisostomo, Daniel R. Meldrum

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background. Preconditioning is injury-induced protection from subsequent insult. Recent data indicates that males have lower preconditioning thresholds compared to females. Therefore, we hypothesized that testosterone may mediate the lower preconditioning threshold observed in males. Materials and methods. Adult normal and castrated male Sprague-Dawley rats (n = 4-5) were given intraperitoneal (i.p.) injections of 125 or 500 μg/kg Salmonella typhimurium lipopolysaccharide (ETX) or 0.4 ml normal saline (NS). Another i.p. injection of 500 μg/kg ETX (injury dose) was given 24 h later. After 6 h, myocardial function was evaluated via the Langendorff perfusion model. Shams received only NS, while non-preconditioned rats (PC-) received NS followed by the 500 μg/kg ETX injury dose. Preconditioned rats received injections of 125 μg/kg ETX (PC +125) or 500 μg/kg ETX (PC +500), followed by the 500 μg/kg ETX injury dose. Results. Normal PC +125 and PC +500 males were preconditioned and maintained cardiac function similar to shams (P > 0.05). Castrated PC +125 and PC +500 males were also preconditioned and maintained cardiac function similar to castrated shams (P > 0.05). Conversely, both normal and castrated PC-males showed significantly decreased cardiac function compared to shams (P < 0.05). Conclusions. Endogenous testosterone does not mediate the lower preconditioning threshold in males.

Original languageEnglish
Pages (from-to)86-90
Number of pages5
JournalJournal of Surgical Research
Volume131
Issue number1
DOIs
StatePublished - Mar 2006

Fingerprint

Testosterone
Wounds and Injuries
Intraperitoneal Injections
Salmonella typhimurium
Sprague Dawley Rats
Lipopolysaccharides
Perfusion
Injections

Keywords

  • Acute injury
  • Cardiac
  • Gender
  • Inflammation

ASJC Scopus subject areas

  • Surgery

Cite this

Nelson, N. T., Mahomed, A. J., Pitcher, J. M., Tsai, B. M., Wang, M., Mahomed, Y., ... Meldrum, D. R. (2006). Does endogenous testosterone mediate the lower preconditioning threshold in males? Journal of Surgical Research, 131(1), 86-90. https://doi.org/10.1016/j.jss.2005.07.039

Does endogenous testosterone mediate the lower preconditioning threshold in males? / Nelson, Nicholas T.; Mahomed, Adam J.; Pitcher, Jeffrey M.; Tsai, Ben M.; Wang, Meijing; Mahomed, Yousuf; Brown, John; Crisostomo, Paul; Meldrum, Daniel R.

In: Journal of Surgical Research, Vol. 131, No. 1, 03.2006, p. 86-90.

Research output: Contribution to journalArticle

Nelson, NT, Mahomed, AJ, Pitcher, JM, Tsai, BM, Wang, M, Mahomed, Y, Brown, J, Crisostomo, P & Meldrum, DR 2006, 'Does endogenous testosterone mediate the lower preconditioning threshold in males?', Journal of Surgical Research, vol. 131, no. 1, pp. 86-90. https://doi.org/10.1016/j.jss.2005.07.039
Nelson, Nicholas T. ; Mahomed, Adam J. ; Pitcher, Jeffrey M. ; Tsai, Ben M. ; Wang, Meijing ; Mahomed, Yousuf ; Brown, John ; Crisostomo, Paul ; Meldrum, Daniel R. / Does endogenous testosterone mediate the lower preconditioning threshold in males?. In: Journal of Surgical Research. 2006 ; Vol. 131, No. 1. pp. 86-90.
@article{6d9323026f3840d39411fb955e55bb23,
title = "Does endogenous testosterone mediate the lower preconditioning threshold in males?",
abstract = "Background. Preconditioning is injury-induced protection from subsequent insult. Recent data indicates that males have lower preconditioning thresholds compared to females. Therefore, we hypothesized that testosterone may mediate the lower preconditioning threshold observed in males. Materials and methods. Adult normal and castrated male Sprague-Dawley rats (n = 4-5) were given intraperitoneal (i.p.) injections of 125 or 500 μg/kg Salmonella typhimurium lipopolysaccharide (ETX) or 0.4 ml normal saline (NS). Another i.p. injection of 500 μg/kg ETX (injury dose) was given 24 h later. After 6 h, myocardial function was evaluated via the Langendorff perfusion model. Shams received only NS, while non-preconditioned rats (PC-) received NS followed by the 500 μg/kg ETX injury dose. Preconditioned rats received injections of 125 μg/kg ETX (PC +125) or 500 μg/kg ETX (PC +500), followed by the 500 μg/kg ETX injury dose. Results. Normal PC +125 and PC +500 males were preconditioned and maintained cardiac function similar to shams (P > 0.05). Castrated PC +125 and PC +500 males were also preconditioned and maintained cardiac function similar to castrated shams (P > 0.05). Conversely, both normal and castrated PC-males showed significantly decreased cardiac function compared to shams (P < 0.05). Conclusions. Endogenous testosterone does not mediate the lower preconditioning threshold in males.",
keywords = "Acute injury, Cardiac, Gender, Inflammation",
author = "Nelson, {Nicholas T.} and Mahomed, {Adam J.} and Pitcher, {Jeffrey M.} and Tsai, {Ben M.} and Meijing Wang and Yousuf Mahomed and John Brown and Paul Crisostomo and Meldrum, {Daniel R.}",
year = "2006",
month = "3",
doi = "10.1016/j.jss.2005.07.039",
language = "English",
volume = "131",
pages = "86--90",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Does endogenous testosterone mediate the lower preconditioning threshold in males?

AU - Nelson, Nicholas T.

AU - Mahomed, Adam J.

AU - Pitcher, Jeffrey M.

AU - Tsai, Ben M.

AU - Wang, Meijing

AU - Mahomed, Yousuf

AU - Brown, John

AU - Crisostomo, Paul

AU - Meldrum, Daniel R.

PY - 2006/3

Y1 - 2006/3

N2 - Background. Preconditioning is injury-induced protection from subsequent insult. Recent data indicates that males have lower preconditioning thresholds compared to females. Therefore, we hypothesized that testosterone may mediate the lower preconditioning threshold observed in males. Materials and methods. Adult normal and castrated male Sprague-Dawley rats (n = 4-5) were given intraperitoneal (i.p.) injections of 125 or 500 μg/kg Salmonella typhimurium lipopolysaccharide (ETX) or 0.4 ml normal saline (NS). Another i.p. injection of 500 μg/kg ETX (injury dose) was given 24 h later. After 6 h, myocardial function was evaluated via the Langendorff perfusion model. Shams received only NS, while non-preconditioned rats (PC-) received NS followed by the 500 μg/kg ETX injury dose. Preconditioned rats received injections of 125 μg/kg ETX (PC +125) or 500 μg/kg ETX (PC +500), followed by the 500 μg/kg ETX injury dose. Results. Normal PC +125 and PC +500 males were preconditioned and maintained cardiac function similar to shams (P > 0.05). Castrated PC +125 and PC +500 males were also preconditioned and maintained cardiac function similar to castrated shams (P > 0.05). Conversely, both normal and castrated PC-males showed significantly decreased cardiac function compared to shams (P < 0.05). Conclusions. Endogenous testosterone does not mediate the lower preconditioning threshold in males.

AB - Background. Preconditioning is injury-induced protection from subsequent insult. Recent data indicates that males have lower preconditioning thresholds compared to females. Therefore, we hypothesized that testosterone may mediate the lower preconditioning threshold observed in males. Materials and methods. Adult normal and castrated male Sprague-Dawley rats (n = 4-5) were given intraperitoneal (i.p.) injections of 125 or 500 μg/kg Salmonella typhimurium lipopolysaccharide (ETX) or 0.4 ml normal saline (NS). Another i.p. injection of 500 μg/kg ETX (injury dose) was given 24 h later. After 6 h, myocardial function was evaluated via the Langendorff perfusion model. Shams received only NS, while non-preconditioned rats (PC-) received NS followed by the 500 μg/kg ETX injury dose. Preconditioned rats received injections of 125 μg/kg ETX (PC +125) or 500 μg/kg ETX (PC +500), followed by the 500 μg/kg ETX injury dose. Results. Normal PC +125 and PC +500 males were preconditioned and maintained cardiac function similar to shams (P > 0.05). Castrated PC +125 and PC +500 males were also preconditioned and maintained cardiac function similar to castrated shams (P > 0.05). Conversely, both normal and castrated PC-males showed significantly decreased cardiac function compared to shams (P < 0.05). Conclusions. Endogenous testosterone does not mediate the lower preconditioning threshold in males.

KW - Acute injury

KW - Cardiac

KW - Gender

KW - Inflammation

UR - http://www.scopus.com/inward/record.url?scp=32444435264&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=32444435264&partnerID=8YFLogxK

U2 - 10.1016/j.jss.2005.07.039

DO - 10.1016/j.jss.2005.07.039

M3 - Article

VL - 131

SP - 86

EP - 90

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 1

ER -