DON, CONV and DONV. I. Inhibition of L asparagine synthetase in vitro

H. N. Jayaram, D. A. Cooney, H. A. Milman, E. R. Homan, R. J. Rosenbluth

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The inhibition in vitro of L asparagine synthetase (L glutamine hydrolyzing, EC 6.3.5.4) from leukemia 5178Y rendered resistant to L asparaginase (L5178Y/AR), and from mouse pancreas by the ketoamine acids DON (L DON; 6 diazo 5 oxo L norleucine), CONV (L CONV; 2 amino 5 chlorolevulinic acid; 5 chloro 4 oxo L norvaline) and DONV (L DONV; 5 diazo 4 oxo L norvaline) was investigated using both L glutamine and ammonium chloride as substrates. At a concentration of 1 mM, DON and CONV almost completely inhibited the utilization in vitro of L glutamine by L asparagine synthetase of L5178Y/AR and of mouse pancreas, whereas DONV inhibited both enzymes only by 50 per cent. DON, CONV and DONV did not affect the utilization in vitro of ammonium chloride by L asparagine synthetase of L5178Y/AR while DON and CONV modestly inhibited the utilization of ammonium chloride by the pancreatic enzyme. The inhibition produced by DONV was fully and rapidly reversed by dialysis, whereas that produced by DON and CONV was essentially irreversible. The utilization in vitro of L glutamine by eight rat liver amidotransferase was strongly inhibited by DON and CONV, while DONV exerted modest inhibition on only two of these enzymes. In a survey of other enzymes which use L asparagine and L glutamine as substrates, DONV was shown to be the best inhibitor of L asparaginase utilizing enzymes. DON was the best inhibitor of enzymes utilizing L glutamine and CONV affected both groups of enzymes to a variable degree. DON irreversibly inhibited L asparagine from Erwinia carotovora (EC 3.5.1.1). This enzyme also was found to catalyze the decomposition of DON. From these findings and also from kinetic studies it is clear that all three ketoamine acids are capable of behaving as antagonists of L glutamine in vitro. DON is the most universally active antagonist of this amino acid, followed by CONV, and last by DONV. Similarly, all three agents can function as L asparagine antagonists under appropriate conditions.

Original languageEnglish (US)
Pages (from-to)1571-1582
Number of pages12
JournalBiochemical Pharmacology
Volume25
Issue number14
DOIs
StatePublished - 1976
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology

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    Jayaram, H. N., Cooney, D. A., Milman, H. A., Homan, E. R., & Rosenbluth, R. J. (1976). DON, CONV and DONV. I. Inhibition of L asparagine synthetase in vitro. Biochemical Pharmacology, 25(14), 1571-1582. https://doi.org/10.1016/0014-5793(76)80147-0