Donor-matched comparison of dental pulp stem cells and bone marrow-derived mesenchymal stem cells in a rat model

Daniel L. Alge, Dan Zhou, Lyndsey L. Adams, Brandon K. Wyss, Matthew D. Shadday, Erik J. Woods, T.M. Gabriel Chu, W. Goebel

Research output: Contribution to journalArticle

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Abstract

Dental pulp stem cells (DPSCs) have drawn much interest for the regeneration of mineralized tissues, and several studies have compared DPSCs to bone marrow-derived mesenchymal stem cells (BMMSCs). However, conflicting results, possibly due to donor-associated variability, have been published and the regenerative potential of DPSCs is currently unclear. In the present study we have sought to address this problem using a donor-matched experimental design to robustly compare the biological properties of DPSCs and BMMSCs. All experiments were performed using cells isolated from a single adult Sprague-Dawley rat. Our results show that DPSCs and BMMSCs had similar morphologies and flow cytometry profiles, were capable of forming colonies in vitro and were capable of osteogenic, chondrogenic and adipogenic differentiation. However, quantitative comparisons revealed that DPSCs had a faster population doubling time and a higher percentage of stem/progenitor cells in the population, as determined by clonogenic assays. Furthermore, while both cell populations formed mineral in vitro, DPSCs had significantly higher alkaline phosphatase activity than BMMSCs after 3 weeks in osteogenic medium. These data show several key differences between DPSCs and BMMSCs and support the possibility of using DPSCs for mineralized tissue regeneration.

Original languageEnglish
Pages (from-to)73-81
Number of pages9
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume4
Issue number1
DOIs
StatePublished - Jan 2010

Fingerprint

Dental Pulp
Stem cells
Mesenchymal Stromal Cells
Pulp
Rats
Bone
Stem Cells
Bone Marrow
Regeneration
Population
Tissue regeneration
Minerals
Alkaline Phosphatase
Sprague Dawley Rats
Flow cytometry
Flow Cytometry
Phosphatases
Research Design

Keywords

  • Bone marrow
  • Clonogenicity
  • Colony formation
  • Dental pulp stem cells
  • Differentiation
  • Donor variation
  • Mesenchymal stem cells
  • Proliferation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

Donor-matched comparison of dental pulp stem cells and bone marrow-derived mesenchymal stem cells in a rat model. / Alge, Daniel L.; Zhou, Dan; Adams, Lyndsey L.; Wyss, Brandon K.; Shadday, Matthew D.; Woods, Erik J.; Chu, T.M. Gabriel; Goebel, W.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 4, No. 1, 01.2010, p. 73-81.

Research output: Contribution to journalArticle

Alge, Daniel L. ; Zhou, Dan ; Adams, Lyndsey L. ; Wyss, Brandon K. ; Shadday, Matthew D. ; Woods, Erik J. ; Chu, T.M. Gabriel ; Goebel, W. / Donor-matched comparison of dental pulp stem cells and bone marrow-derived mesenchymal stem cells in a rat model. In: Journal of Tissue Engineering and Regenerative Medicine. 2010 ; Vol. 4, No. 1. pp. 73-81.
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