Dopaminergic parameters in the striatum and substantia nigra of seven strains of mice: Higher density in striatum of CAST compared to BALB mice

J. A. Richter, M. G. Brenneman, Stephen Dlouhy, Bernardino Ghetti

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Tyrosine hydroxylase activity was assayed in microdissected substantia nigra and striata from seven strains of mice (BALB, CBA, YBR, WB, IS, MOLG, and CAST). In the substantia nigra where tyrosine hydroxylase activity is thought to be proportional to dopaminergic neuron number, only CBA had a different (lower) enzyme activity compared with BALB. However in the striatum, tyrosine hydroxylase activity was larger for IS, MOLG and CAST compared with BALB. Further investigation of the CAST striatum showed that dopamine content and dopamine uptake activity were also higher in comparison with BALB. All three dopaminergic parameters were larger because of lower protein levels in the CAST striatum. A lower absolute amount of glutamic acid decarboxylase activity in CAST versus BALB striatum was consistent with the possibility of a smaller CAST striatum. In contrast to dopamine, the serotonin content in CAST striatum was reduced in proportion to the decrease in protein content. We suggest that the CAST striatum is smaller than BALB striatum and is innervated by proportionally fewer serotoninergic terminals, but the amount of dopaminergic innervation of the CAST striatum is not altered by the size of the target.

Original languageEnglish
Pages (from-to)395-400
Number of pages6
JournalNeurochemical Research
Volume20
Issue number4
DOIs
StatePublished - Apr 1995

Fingerprint

Tyrosine 3-Monooxygenase
Substantia Nigra
Dopamine
Inbred CBA Mouse
Glutamate Decarboxylase
Dopaminergic Neurons
Enzyme activity
Neurons
Serotonin
Proteins
Enzymes

Keywords

  • dopamine
  • glutamic acid hydroxylase
  • Mouse strains
  • nigrostriatal pathway
  • serotonin
  • tyrosine hydroxylase

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

@article{27a6c7d5a36249a6b75d62aab3aa472c,
title = "Dopaminergic parameters in the striatum and substantia nigra of seven strains of mice: Higher density in striatum of CAST compared to BALB mice",
abstract = "Tyrosine hydroxylase activity was assayed in microdissected substantia nigra and striata from seven strains of mice (BALB, CBA, YBR, WB, IS, MOLG, and CAST). In the substantia nigra where tyrosine hydroxylase activity is thought to be proportional to dopaminergic neuron number, only CBA had a different (lower) enzyme activity compared with BALB. However in the striatum, tyrosine hydroxylase activity was larger for IS, MOLG and CAST compared with BALB. Further investigation of the CAST striatum showed that dopamine content and dopamine uptake activity were also higher in comparison with BALB. All three dopaminergic parameters were larger because of lower protein levels in the CAST striatum. A lower absolute amount of glutamic acid decarboxylase activity in CAST versus BALB striatum was consistent with the possibility of a smaller CAST striatum. In contrast to dopamine, the serotonin content in CAST striatum was reduced in proportion to the decrease in protein content. We suggest that the CAST striatum is smaller than BALB striatum and is innervated by proportionally fewer serotoninergic terminals, but the amount of dopaminergic innervation of the CAST striatum is not altered by the size of the target.",
keywords = "dopamine, glutamic acid hydroxylase, Mouse strains, nigrostriatal pathway, serotonin, tyrosine hydroxylase",
author = "Richter, {J. A.} and Brenneman, {M. G.} and Stephen Dlouhy and Bernardino Ghetti",
year = "1995",
month = "4",
doi = "10.1007/BF00973093",
language = "English",
volume = "20",
pages = "395--400",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Dopaminergic parameters in the striatum and substantia nigra of seven strains of mice

T2 - Higher density in striatum of CAST compared to BALB mice

AU - Richter, J. A.

AU - Brenneman, M. G.

AU - Dlouhy, Stephen

AU - Ghetti, Bernardino

PY - 1995/4

Y1 - 1995/4

N2 - Tyrosine hydroxylase activity was assayed in microdissected substantia nigra and striata from seven strains of mice (BALB, CBA, YBR, WB, IS, MOLG, and CAST). In the substantia nigra where tyrosine hydroxylase activity is thought to be proportional to dopaminergic neuron number, only CBA had a different (lower) enzyme activity compared with BALB. However in the striatum, tyrosine hydroxylase activity was larger for IS, MOLG and CAST compared with BALB. Further investigation of the CAST striatum showed that dopamine content and dopamine uptake activity were also higher in comparison with BALB. All three dopaminergic parameters were larger because of lower protein levels in the CAST striatum. A lower absolute amount of glutamic acid decarboxylase activity in CAST versus BALB striatum was consistent with the possibility of a smaller CAST striatum. In contrast to dopamine, the serotonin content in CAST striatum was reduced in proportion to the decrease in protein content. We suggest that the CAST striatum is smaller than BALB striatum and is innervated by proportionally fewer serotoninergic terminals, but the amount of dopaminergic innervation of the CAST striatum is not altered by the size of the target.

AB - Tyrosine hydroxylase activity was assayed in microdissected substantia nigra and striata from seven strains of mice (BALB, CBA, YBR, WB, IS, MOLG, and CAST). In the substantia nigra where tyrosine hydroxylase activity is thought to be proportional to dopaminergic neuron number, only CBA had a different (lower) enzyme activity compared with BALB. However in the striatum, tyrosine hydroxylase activity was larger for IS, MOLG and CAST compared with BALB. Further investigation of the CAST striatum showed that dopamine content and dopamine uptake activity were also higher in comparison with BALB. All three dopaminergic parameters were larger because of lower protein levels in the CAST striatum. A lower absolute amount of glutamic acid decarboxylase activity in CAST versus BALB striatum was consistent with the possibility of a smaller CAST striatum. In contrast to dopamine, the serotonin content in CAST striatum was reduced in proportion to the decrease in protein content. We suggest that the CAST striatum is smaller than BALB striatum and is innervated by proportionally fewer serotoninergic terminals, but the amount of dopaminergic innervation of the CAST striatum is not altered by the size of the target.

KW - dopamine

KW - glutamic acid hydroxylase

KW - Mouse strains

KW - nigrostriatal pathway

KW - serotonin

KW - tyrosine hydroxylase

UR - http://www.scopus.com/inward/record.url?scp=0029004679&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029004679&partnerID=8YFLogxK

U2 - 10.1007/BF00973093

DO - 10.1007/BF00973093

M3 - Article

C2 - 7651576

AN - SCOPUS:0029004679

VL - 20

SP - 395

EP - 400

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 4

ER -