Dose analysis of photobiomodulation therapy on osteoblast, osteoclast, and osteocyte

Sungsoo Na, Thuc Nhi TruongVo, Feifei Jiang, Jeffery E. Joll, Yunxia Guo, Achint Utreja, Jie Chen

Research output: Contribution to journalArticle

4 Scopus citations


The objective of this study was to evaluate the effects of varying light doses on the viability and cellular activity of osteoblasts, osteocytes, and osteoclasts. A light application device was developed to apply 940-nm wavelength light from light-emitting diodes on three cultured cells, MC3T3-E1, MLO-A5, and RANKL-treated RAW264.7 cells. The doses (energy density) on cells were 0, 1, 5, and 7.5 J / cm2. The corresponding light power densities at the cell site were 0, 1.67, 8.33, and 12.5 mW / cm2, respectively, and the duration was 10 min. The results showed that the three cell types respond differently to light and their responses were dose dependent. Low-dose treatment (1 J / cm2) enhanced osteoblast proliferation, osteoclast differentiation, and osteoclastic bone resorption activity. Osteocyte proliferation was not affected by both low- and high-dose (5 J / cm2) treatments. While 1 J / cm2 did not affect viability of all three cell types, 5 J / cm2 significantly decreased viability of osteocytes and osteoclasts. Osteoblast viability was negatively impacted by the higher dose (7.5 J / cm2). The findings suggest that optimal doses exist for osteoblast and osteoclast, which can stimulate cell activities, and there is a safe dose range for each type of cell tested.

Original languageEnglish (US)
Article number075008
JournalJournal of Biomedical Optics
Issue number7
StatePublished - Jul 1 2018


  • cell viability
  • differentiation
  • light dose
  • osteoblast
  • osteoclast
  • osteocyte
  • photobiomodulation
  • proliferation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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    Na, S., TruongVo, T. N., Jiang, F., Joll, J. E., Guo, Y., Utreja, A., & Chen, J. (2018). Dose analysis of photobiomodulation therapy on osteoblast, osteoclast, and osteocyte. Journal of Biomedical Optics, 23(7), [075008].