Double-inducible gene activation system for caspase 3 and 9 in epidermis

Viraj R. Shah, Maranke I. Koster, Dennis R. Roop, David M. Spencer, Lei Wei, Qi Li, Robert J. Schwartz, Jiang Chang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Expression of genes with tight and precise temporal and spatial control is desired in a wide variety of applications ranging from cultured cells and transgenic animals to gene therapy. While current inducible systems, such as RU486 and chemical inducers of dimerization (CID), have improved earlier inducible models (Gossen et al., 1995, Science. 268:1766-1769; Wang et al., 1994, Proc Natl Acad Sci USA 91:8180-8184), no single system is perfect at present. One potential drawback of these systems is leakage of transgene expression, causing limitations of each system. We have developed an inducible model containing both RU486 and CID systems, which in addition to inducing caspase activation, has potential applicability specifically to other genes encoding proteins that require a dimerization event for activation. This Double-Inducible Gene Activation System generates two barriers for the target gene expression and protein activation thereby minimizing leakage.

Original languageEnglish
Pages (from-to)194-199
Number of pages6
JournalGenesis
Volume45
Issue number4
DOIs
StatePublished - Apr 2007

Fingerprint

Caspase 9
Dimerization
Epidermis
Caspase 3
Transcriptional Activation
Gene Expression
Genetically Modified Animals
Caspases
Transgenes
Genetic Therapy
Cultured Cells
Proteins

Keywords

  • CID
  • Double-inducible system
  • Inducible gene expression
  • Keratin 14
  • RU486

ASJC Scopus subject areas

  • Genetics

Cite this

Shah, V. R., Koster, M. I., Roop, D. R., Spencer, D. M., Wei, L., Li, Q., ... Chang, J. (2007). Double-inducible gene activation system for caspase 3 and 9 in epidermis. Genesis, 45(4), 194-199. https://doi.org/10.1002/dvg.20288

Double-inducible gene activation system for caspase 3 and 9 in epidermis. / Shah, Viraj R.; Koster, Maranke I.; Roop, Dennis R.; Spencer, David M.; Wei, Lei; Li, Qi; Schwartz, Robert J.; Chang, Jiang.

In: Genesis, Vol. 45, No. 4, 04.2007, p. 194-199.

Research output: Contribution to journalArticle

Shah, VR, Koster, MI, Roop, DR, Spencer, DM, Wei, L, Li, Q, Schwartz, RJ & Chang, J 2007, 'Double-inducible gene activation system for caspase 3 and 9 in epidermis', Genesis, vol. 45, no. 4, pp. 194-199. https://doi.org/10.1002/dvg.20288
Shah VR, Koster MI, Roop DR, Spencer DM, Wei L, Li Q et al. Double-inducible gene activation system for caspase 3 and 9 in epidermis. Genesis. 2007 Apr;45(4):194-199. https://doi.org/10.1002/dvg.20288
Shah, Viraj R. ; Koster, Maranke I. ; Roop, Dennis R. ; Spencer, David M. ; Wei, Lei ; Li, Qi ; Schwartz, Robert J. ; Chang, Jiang. / Double-inducible gene activation system for caspase 3 and 9 in epidermis. In: Genesis. 2007 ; Vol. 45, No. 4. pp. 194-199.
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