Doxycycline-encapsulated nanotube-modified dentin adhesives

Sabrina Feitosa, J. Palasuk, K. Kamocki, S. Geraldeli, Richard Gregory, Jeffrey Platt, L. Windsor, M. C. Bottino

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

This article presents details of fabrication, biological activity (i.e., anti-matrix metalloproteinase [anti-MMP] inhibition), cytocompatibility, and bonding characteristics to dentin of a unique doxycycline (DOX)-encapsulated halloysite nanotube (HNT)-modified adhesive. We tested the hypothesis that the release of DOX from the DOX-encapsulated nanotube-modified adhesive can effectively inhibit MMP activity. We incorporated nanotubes, encapsulated or not with DOX, into the adhesive resin of a commercially available bonding system (Scotchbond Multi-Purpose [SBMP]). The following groups were tested: unmodified SBMP (control), SBMP with nanotubes (HNT), and DOX-encapsulated nanotube-modified adhesive (HNT+DOX). Changes in degree of conversion (DC) and microtensile bond strength were evaluated. Cytotoxicity was examined on human dental pulp stem cells (hDPSCs). To prove the successful encapsulation of DOX within the adhesives - but, more important, to support the hypothesis that the HNT+DOX adhesive would release DOX at subantimicrobial levels - we tested the antimicrobial activity of synthesized adhesives and the DOX-containing eluates against Streptococcus mutans through agar diffusion assays. Anti-MMP properties were assessed via β-casein cleavage assays. Increasing curing times (10, 20, 40 sec) led to increased DC values. There were no statistically significant differences (p >.05) in DC within each increasing curing time between the modified adhesives compared to SBMP. No statistically significant differences in microtensile bond strength were noted. None of the adhesives eluates were cytotoxic to the human dental pulp stem cells. A significant growth inhibition of S. mutans by direct contact illustrates successful encapsulation of DOX into the experimental adhesive. More important, DOX-containing eluates promoted inhibition of MMP-1 activity when compared to the control. Collectively, our findings provide a solid background for further testing of encapsulated MMP inhibitors into the synthesis of therapeutic adhesives that may enhance the longevity of hybrid layers and the overall clinical performance of adhesively bonded resin composite restorations.

Original languageEnglish
Pages (from-to)1270-1276
Number of pages7
JournalJournal of Dental Research
Volume93
Issue number12
DOIs
StatePublished - Dec 25 2014

Fingerprint

Nanotubes
Doxycycline
Dentin
Adhesives
Matrix Metalloproteinases
Dental Pulp
Streptococcus mutans
Stem Cells
Matrix Metalloproteinase Inhibitors
Composite Resins
Caseins
Agar

Keywords

  • biocompatible materials
  • bonding
  • collagen
  • drug delivery systems
  • matrix metalloproteinase
  • stem cells

ASJC Scopus subject areas

  • Dentistry(all)
  • Medicine(all)

Cite this

Doxycycline-encapsulated nanotube-modified dentin adhesives. / Feitosa, Sabrina; Palasuk, J.; Kamocki, K.; Geraldeli, S.; Gregory, Richard; Platt, Jeffrey; Windsor, L.; Bottino, M. C.

In: Journal of Dental Research, Vol. 93, No. 12, 25.12.2014, p. 1270-1276.

Research output: Contribution to journalArticle

Feitosa, Sabrina ; Palasuk, J. ; Kamocki, K. ; Geraldeli, S. ; Gregory, Richard ; Platt, Jeffrey ; Windsor, L. ; Bottino, M. C. / Doxycycline-encapsulated nanotube-modified dentin adhesives. In: Journal of Dental Research. 2014 ; Vol. 93, No. 12. pp. 1270-1276.
@article{9202d7581ed64762821ec271c8ebdc7c,
title = "Doxycycline-encapsulated nanotube-modified dentin adhesives",
abstract = "This article presents details of fabrication, biological activity (i.e., anti-matrix metalloproteinase [anti-MMP] inhibition), cytocompatibility, and bonding characteristics to dentin of a unique doxycycline (DOX)-encapsulated halloysite nanotube (HNT)-modified adhesive. We tested the hypothesis that the release of DOX from the DOX-encapsulated nanotube-modified adhesive can effectively inhibit MMP activity. We incorporated nanotubes, encapsulated or not with DOX, into the adhesive resin of a commercially available bonding system (Scotchbond Multi-Purpose [SBMP]). The following groups were tested: unmodified SBMP (control), SBMP with nanotubes (HNT), and DOX-encapsulated nanotube-modified adhesive (HNT+DOX). Changes in degree of conversion (DC) and microtensile bond strength were evaluated. Cytotoxicity was examined on human dental pulp stem cells (hDPSCs). To prove the successful encapsulation of DOX within the adhesives - but, more important, to support the hypothesis that the HNT+DOX adhesive would release DOX at subantimicrobial levels - we tested the antimicrobial activity of synthesized adhesives and the DOX-containing eluates against Streptococcus mutans through agar diffusion assays. Anti-MMP properties were assessed via β-casein cleavage assays. Increasing curing times (10, 20, 40 sec) led to increased DC values. There were no statistically significant differences (p >.05) in DC within each increasing curing time between the modified adhesives compared to SBMP. No statistically significant differences in microtensile bond strength were noted. None of the adhesives eluates were cytotoxic to the human dental pulp stem cells. A significant growth inhibition of S. mutans by direct contact illustrates successful encapsulation of DOX into the experimental adhesive. More important, DOX-containing eluates promoted inhibition of MMP-1 activity when compared to the control. Collectively, our findings provide a solid background for further testing of encapsulated MMP inhibitors into the synthesis of therapeutic adhesives that may enhance the longevity of hybrid layers and the overall clinical performance of adhesively bonded resin composite restorations.",
keywords = "biocompatible materials, bonding, collagen, drug delivery systems, matrix metalloproteinase, stem cells",
author = "Sabrina Feitosa and J. Palasuk and K. Kamocki and S. Geraldeli and Richard Gregory and Jeffrey Platt and L. Windsor and Bottino, {M. C.}",
year = "2014",
month = "12",
day = "25",
doi = "10.1177/0022034514549997",
language = "English",
volume = "93",
pages = "1270--1276",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",
number = "12",

}

TY - JOUR

T1 - Doxycycline-encapsulated nanotube-modified dentin adhesives

AU - Feitosa, Sabrina

AU - Palasuk, J.

AU - Kamocki, K.

AU - Geraldeli, S.

AU - Gregory, Richard

AU - Platt, Jeffrey

AU - Windsor, L.

AU - Bottino, M. C.

PY - 2014/12/25

Y1 - 2014/12/25

N2 - This article presents details of fabrication, biological activity (i.e., anti-matrix metalloproteinase [anti-MMP] inhibition), cytocompatibility, and bonding characteristics to dentin of a unique doxycycline (DOX)-encapsulated halloysite nanotube (HNT)-modified adhesive. We tested the hypothesis that the release of DOX from the DOX-encapsulated nanotube-modified adhesive can effectively inhibit MMP activity. We incorporated nanotubes, encapsulated or not with DOX, into the adhesive resin of a commercially available bonding system (Scotchbond Multi-Purpose [SBMP]). The following groups were tested: unmodified SBMP (control), SBMP with nanotubes (HNT), and DOX-encapsulated nanotube-modified adhesive (HNT+DOX). Changes in degree of conversion (DC) and microtensile bond strength were evaluated. Cytotoxicity was examined on human dental pulp stem cells (hDPSCs). To prove the successful encapsulation of DOX within the adhesives - but, more important, to support the hypothesis that the HNT+DOX adhesive would release DOX at subantimicrobial levels - we tested the antimicrobial activity of synthesized adhesives and the DOX-containing eluates against Streptococcus mutans through agar diffusion assays. Anti-MMP properties were assessed via β-casein cleavage assays. Increasing curing times (10, 20, 40 sec) led to increased DC values. There were no statistically significant differences (p >.05) in DC within each increasing curing time between the modified adhesives compared to SBMP. No statistically significant differences in microtensile bond strength were noted. None of the adhesives eluates were cytotoxic to the human dental pulp stem cells. A significant growth inhibition of S. mutans by direct contact illustrates successful encapsulation of DOX into the experimental adhesive. More important, DOX-containing eluates promoted inhibition of MMP-1 activity when compared to the control. Collectively, our findings provide a solid background for further testing of encapsulated MMP inhibitors into the synthesis of therapeutic adhesives that may enhance the longevity of hybrid layers and the overall clinical performance of adhesively bonded resin composite restorations.

AB - This article presents details of fabrication, biological activity (i.e., anti-matrix metalloproteinase [anti-MMP] inhibition), cytocompatibility, and bonding characteristics to dentin of a unique doxycycline (DOX)-encapsulated halloysite nanotube (HNT)-modified adhesive. We tested the hypothesis that the release of DOX from the DOX-encapsulated nanotube-modified adhesive can effectively inhibit MMP activity. We incorporated nanotubes, encapsulated or not with DOX, into the adhesive resin of a commercially available bonding system (Scotchbond Multi-Purpose [SBMP]). The following groups were tested: unmodified SBMP (control), SBMP with nanotubes (HNT), and DOX-encapsulated nanotube-modified adhesive (HNT+DOX). Changes in degree of conversion (DC) and microtensile bond strength were evaluated. Cytotoxicity was examined on human dental pulp stem cells (hDPSCs). To prove the successful encapsulation of DOX within the adhesives - but, more important, to support the hypothesis that the HNT+DOX adhesive would release DOX at subantimicrobial levels - we tested the antimicrobial activity of synthesized adhesives and the DOX-containing eluates against Streptococcus mutans through agar diffusion assays. Anti-MMP properties were assessed via β-casein cleavage assays. Increasing curing times (10, 20, 40 sec) led to increased DC values. There were no statistically significant differences (p >.05) in DC within each increasing curing time between the modified adhesives compared to SBMP. No statistically significant differences in microtensile bond strength were noted. None of the adhesives eluates were cytotoxic to the human dental pulp stem cells. A significant growth inhibition of S. mutans by direct contact illustrates successful encapsulation of DOX into the experimental adhesive. More important, DOX-containing eluates promoted inhibition of MMP-1 activity when compared to the control. Collectively, our findings provide a solid background for further testing of encapsulated MMP inhibitors into the synthesis of therapeutic adhesives that may enhance the longevity of hybrid layers and the overall clinical performance of adhesively bonded resin composite restorations.

KW - biocompatible materials

KW - bonding

KW - collagen

KW - drug delivery systems

KW - matrix metalloproteinase

KW - stem cells

UR - http://www.scopus.com/inward/record.url?scp=84911947835&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84911947835&partnerID=8YFLogxK

U2 - 10.1177/0022034514549997

DO - 10.1177/0022034514549997

M3 - Article

C2 - 25201918

AN - SCOPUS:84911947835

VL - 93

SP - 1270

EP - 1276

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

IS - 12

ER -