Drosophila forkhead homologues are expressed in a lineage-restricted manner in human hematopoietic cells

Robert Hromas, Jason Moore, Timothy Johnston, Carolynn Socha, Michael Klemsz

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The forkhead gene (FKH) regulates morphogenesis in Drosophila. It is the prototype of a new family of transcriptional activators. Partially degenerate oligonucleotides to two conserved amino acid sequences of this family were used to prime a polymerase chain reaction (PCR) amplification of HEL cell cDNA. Two unique clones, designated H3 and HS, were isolated that contained homologies to FKH. A third novel clone, 5-3, was isolated by low stringency screening of a chronic myelogenous leukemia cDNA library using H8 probe. H3 and 5-3 are preferentially expressed in restricted hematopoietic lineages, while the expression of H8 was ubiquitous. Southern analysis showed that FKH 5-3 is conserved through yeast, which is rare among tissue-specific transcription factors. The H3 and 5-3 clones provide evidence that FKH family members are present in a tissue-restricted manner in humans.

Original languageEnglish
Pages (from-to)2854-2859
Number of pages6
JournalBlood
Volume81
Issue number11
StatePublished - Jun 1 1993

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Drosophila
Genes
Clone Cells
Tissue
Polymerase chain reaction
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Gene Library
Morphogenesis
Oligonucleotides
Yeast
Amplification
Amino Acid Sequence
Screening
Transcription Factors
Complementary DNA
Yeasts
Amino Acids
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Hematology

Cite this

Drosophila forkhead homologues are expressed in a lineage-restricted manner in human hematopoietic cells. / Hromas, Robert; Moore, Jason; Johnston, Timothy; Socha, Carolynn; Klemsz, Michael.

In: Blood, Vol. 81, No. 11, 01.06.1993, p. 2854-2859.

Research output: Contribution to journalArticle

Hromas, R, Moore, J, Johnston, T, Socha, C & Klemsz, M 1993, 'Drosophila forkhead homologues are expressed in a lineage-restricted manner in human hematopoietic cells', Blood, vol. 81, no. 11, pp. 2854-2859.
Hromas, Robert ; Moore, Jason ; Johnston, Timothy ; Socha, Carolynn ; Klemsz, Michael. / Drosophila forkhead homologues are expressed in a lineage-restricted manner in human hematopoietic cells. In: Blood. 1993 ; Vol. 81, No. 11. pp. 2854-2859.
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