Drosophila forkhead homologues are expressed in CD34+HLA-DR- primitive human hematopoietic progenitors

Robert Hromas, Michael Klemsz, Lakshmi Amaravadi, Tricia Hufford, Irene Huang, Alpana Desai, Edward Srour, Edward Bruno, Ronald Hoffman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The Forkhead gene (FKH) regulates morphogenesis in Drosophila. It is the prototype of a new family of transcriptional activators. We used the polymerase chain reaction (PCR) to analyze the expression pattern of this new transcriptional regulatory gene family in primitive hematopoeitic progenitors. Partially degenerate oligonucleotides to two conserved amino acid sequences of this family were used to prime a PCR amplification of cDNA synthesized from CD34+HLA-DR- hematopoietic cells. Known and novel FKH genes were found to be expressed in these cells.

Original languageEnglish
Pages (from-to)439-444
Number of pages6
JournalLeukemia and Lymphoma
Volume15
Issue number5-6
DOIs
StatePublished - 1994

Fingerprint

HLA-DR Antigens
Drosophila
Genes
Polymerase Chain Reaction
Regulator Genes
Morphogenesis
Oligonucleotides
Amino Acid Sequence
Complementary DNA

Keywords

  • Drosophila forkhead homologues
  • Forkhead
  • Transcription regulators

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Drosophila forkhead homologues are expressed in CD34+HLA-DR- primitive human hematopoietic progenitors. / Hromas, Robert; Klemsz, Michael; Amaravadi, Lakshmi; Hufford, Tricia; Huang, Irene; Desai, Alpana; Srour, Edward; Bruno, Edward; Hoffman, Ronald.

In: Leukemia and Lymphoma, Vol. 15, No. 5-6, 1994, p. 439-444.

Research output: Contribution to journalArticle

Hromas, Robert ; Klemsz, Michael ; Amaravadi, Lakshmi ; Hufford, Tricia ; Huang, Irene ; Desai, Alpana ; Srour, Edward ; Bruno, Edward ; Hoffman, Ronald. / Drosophila forkhead homologues are expressed in CD34+HLA-DR- primitive human hematopoietic progenitors. In: Leukemia and Lymphoma. 1994 ; Vol. 15, No. 5-6. pp. 439-444.
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AU - Huang, Irene

AU - Desai, Alpana

AU - Srour, Edward

AU - Bruno, Edward

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