Drosophila methyltransferase activity and the repair of alkylated DNA

Sami N. Guzder, Mark Kelley, Walter A. Deutsch

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The biochemical mechanism and developmental expression for the repair of alkylated DNA has been characterized from Drosophila. As in other organisms, the correction of O6-methylguanine in Drosophila was found to involve the transfer of a methyl group from DNA to a protein cysteine residue. Two methylated proteins with subunit molecular weights of 30 kDa and 19 kDa were identified following incubation with [3H]-methylated substrate DNA and denaturing polyacrylamide gel electrophoresis. Identical molecular weights were found for the unmethylated forms of protein through their reaction to an antibody prepared againts the 19 kDa Escherichia coli methyltransferase. Both Drosophila proteins are serologically reactive in adult males and females and most of the other developmental stages tested, with embryos representing the possible exception. The Drosophila proteins do not appear to be induced by sublethal exposures to alkylating agent.

Original languageEnglish (US)
Pages (from-to)143-153
Number of pages11
JournalMutation Research - DNA Repair
Volume255
Issue number2
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Drosophila Proteins
Methyltransferases
DNA Repair
Drosophila
Repair
Molecular Weight
Alkylating Agents
DNA
Protein Subunits
Molecular weight
Cysteine
Polyacrylamide Gel Electrophoresis
Proteins
Embryonic Structures
Escherichia coli
Electrophoresis
Antibodies
Substrates
O-(6)-methylguanine

Keywords

  • (Drosophila melanogaster)
  • Alkylated DNA, repair
  • Alkyltransferases
  • DNA repair
  • O-Methylguanine

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Toxicology

Cite this

Drosophila methyltransferase activity and the repair of alkylated DNA. / Guzder, Sami N.; Kelley, Mark; Deutsch, Walter A.

In: Mutation Research - DNA Repair, Vol. 255, No. 2, 1991, p. 143-153.

Research output: Contribution to journalArticle

Guzder, Sami N. ; Kelley, Mark ; Deutsch, Walter A. / Drosophila methyltransferase activity and the repair of alkylated DNA. In: Mutation Research - DNA Repair. 1991 ; Vol. 255, No. 2. pp. 143-153.
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