Drug-releasing nano-engineered titanium implants

therapeutic efficacy in 3D cell culture model, controlled release and stability

Karan Gulati, Masakazu Kogawa, Matthew Prideaux, David M. Findlay, Gerald J. Atkins, Dusan Losic

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

There is an ongoing demand for new approaches for treating localized bone pathologies. Here we propose a new strategy for treatment of such conditions, via local delivery of hormones/drugs to the trauma site using drug releasing nano-engineered implants. The proposed implants were prepared in the form of small Ti wires/needles with a nano-engineered oxide layer composed of array of titania nanotubes (TNTs). TNTs implants were inserted into a 3D collagen gel matrix containing human osteoblast-like, and the results confirmed cell migration onto the implants and their attachment and spread. To investigate therapeutic efficacy, TNTs/Ti wires loaded with parathyroid hormone (PTH), an approved anabolic therapeutic for the treatment of severe bone fractures, were inserted into 3D gels containing osteoblast-like cells. Gene expression studies revealed a suppression of SOST (sclerostin) and an increase in RANKL (receptor activator of nuclear factor kappa-B ligand) mRNA expression, confirming the release of PTH from TNTs at concentrations sufficient to alter cell function. The performance of the TNTs wire implants using an example of a drug needed at relatively higher concentrations, the anti-inflammatory drug indomethacin, is also demonstrated. Finally, the mechanical stability of the prepared implants was tested by their insertion into bovine trabecular bone cores ex vivo followed by retrieval, which confirmed the robustness of the TNT structures. This study provides proof of principle for the suitability of the TNT/Ti wire implants for localized bone therapy, which can be customized to cater for specific therapeutic requirements.

Original languageEnglish (US)
Pages (from-to)831-840
Number of pages10
JournalMaterials Science and Engineering C
Volume69
DOIs
StatePublished - Dec 1 2016
Externally publishedYes

Fingerprint

releasing
Titanium
Cell culture
Nanotubes
nanotubes
drugs
titanium
bones
hormones
Pharmaceutical Preparations
Bone
wire
Wire
osteoblasts
Osteoblasts
Parathyroid Hormone
Gels
cells
gels
RANK Ligand

Keywords

  • 3D cell culture
  • Bone implants
  • Local drug delivery
  • Parathyroid hormone
  • TiO nanotubes
  • Titanium

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Drug-releasing nano-engineered titanium implants : therapeutic efficacy in 3D cell culture model, controlled release and stability. / Gulati, Karan; Kogawa, Masakazu; Prideaux, Matthew; Findlay, David M.; Atkins, Gerald J.; Losic, Dusan.

In: Materials Science and Engineering C, Vol. 69, 01.12.2016, p. 831-840.

Research output: Contribution to journalArticle

Gulati, Karan ; Kogawa, Masakazu ; Prideaux, Matthew ; Findlay, David M. ; Atkins, Gerald J. ; Losic, Dusan. / Drug-releasing nano-engineered titanium implants : therapeutic efficacy in 3D cell culture model, controlled release and stability. In: Materials Science and Engineering C. 2016 ; Vol. 69. pp. 831-840.
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