Drug Response of Head and Neck Tumors in Native-State Histoculture

K. T. Robbins, N. M. Varki, Anna Maria Storniolo, H. Hoffman, R. M. Hoffman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We describe a chemosensitivity testing of head and neck tumors, in which a native-state histoculture, ie, a three-dimensional culture system that maintains important in vivo properties, including tissue architecture, was used. Fifteen specimens of head and neck tumors were evaluated for sensitivity to the following drugs: Cisplatin (DDP) at concentrations of 1.5, 15, and 37.5 μg/mL; fluorouracil at concentrations of 4.0, 40, and 100μg/mL; and combinations of cisplatin and fluorouracil in corresponding doses. Growth and measurement of drug responses were successfully completed in 10 specimens (five others were contaminated, four of them prior to instituting rigorous antibiotic washes). The results indicated cisplatin sensitivity in five of 10 patients; fluorouracil sensitivity in four of 10 patients; and fluorouracil-cisplatin sensitivity in seven of eight patients. Our preliminary results indicate that the native-state histoculture technique is feasible to test chemosensitivity of head and neck tumors.

Original languageEnglish (US)
Pages (from-to)83-86
Number of pages4
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume117
Issue number1
DOIs
StatePublished - 1991
Externally publishedYes

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Fluorouracil
Cisplatin
Neck
Head
Pharmaceutical Preparations
Neoplasms
Anti-Bacterial Agents
Growth

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery

Cite this

Drug Response of Head and Neck Tumors in Native-State Histoculture. / Robbins, K. T.; Varki, N. M.; Storniolo, Anna Maria; Hoffman, H.; Hoffman, R. M.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 117, No. 1, 1991, p. 83-86.

Research output: Contribution to journalArticle

Robbins, K. T. ; Varki, N. M. ; Storniolo, Anna Maria ; Hoffman, H. ; Hoffman, R. M. / Drug Response of Head and Neck Tumors in Native-State Histoculture. In: Archives of Otolaryngology - Head and Neck Surgery. 1991 ; Vol. 117, No. 1. pp. 83-86.
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