Drug Response of Head and Neck Tumors in Native-State Histoculture

K. T. Robbins, N. M. Varki, A. M. Storniolo, H. Hoffman, R. M. Hoffman

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Abstract

We describe a chemosensitivity testing of head and neck tumors, in which a native-state histoculture, ie, a three-dimensional culture system that maintains important in vivo properties, including tissue architecture, was used. Fifteen specimens of head and neck tumors were evaluated for sensitivity to the following drugs: Cisplatin (DDP) at concentrations of 1.5, 15, and 37.5 μg/mL; fluorouracil at concentrations of 4.0, 40, and 100μg/mL; and combinations of cisplatin and fluorouracil in corresponding doses. Growth and measurement of drug responses were successfully completed in 10 specimens (five others were contaminated, four of them prior to instituting rigorous antibiotic washes). The results indicated cisplatin sensitivity in five of 10 patients; fluorouracil sensitivity in four of 10 patients; and fluorouracil-cisplatin sensitivity in seven of eight patients. Our preliminary results indicate that the native-state histoculture technique is feasible to test chemosensitivity of head and neck tumors.

Original languageEnglish (US)
Pages (from-to)83-86
Number of pages4
JournalArchives of Otolaryngology--Head and Neck Surgery
Volume117
Issue number1
DOIs
StatePublished - Jan 1991

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ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

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