Dual lineage-specific expression of Sox17 during mouse embryogenesis

Eunyoung Choi, Marine R C Kraus, Laurence A. Lemaire, Momoko Yoshimoto, Sasidhar Vemula, Leah A. Potter, Elisabetta Manduchi, Christian J. Stoeckert, Anne Grapin-Botton, Mark A. Magnuson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Sox17 is essential for both endoderm development and fetal hematopoietic stem cell (HSC) maintenance. While endoderm- derived organs are well known to originate from Sox17-expressing cells, it is less certain whether fetal HSCs also originate from Sox17-expressing cells. By generating a Sox17 GFPCre allele and using it to assess the fate of Sox17-expressing cells during embryogenesis, we confirmed that both endodermal and a part of definitive hematopoietic cells are derived from Sox17-positive cells. Prior to E9.5, the expression of Sox17 is restricted to the endoderm lineage. However, at E9.5 Sox17 is expressed in the endothelial cells (ECs) at the para-aortic splanchnopleural region that contribute to the formation of HSCs at a later stage. The identification of two distinct progenitor cell populations that express Sox17 at E9.5 was confirmed using fluorescence-activated cell sorting together with RNA-Seq to determine the gene expression profiles of the two cell populations. Interestingly, this analysis revealed differences in the RNA processing of the Sox17 mRNA during embryogenesis. Taken together, these results indicate that Sox17 is expressed in progenitor cells derived from two different germ layers, further demonstrating the complex expression pattern of this gene and suggesting caution when using Sox17 as a lineage-specific marker.

Original languageEnglish
Pages (from-to)2297-2308
Number of pages12
JournalStem Cells
Volume30
Issue number10
DOIs
StatePublished - Oct 2012

Fingerprint

Embryonic Development
Endoderm
Stem Cells
Fetal Stem Cells
RNA
Germ Layers
Hematopoietic Stem Cells
Transcriptome
Population
Flow Cytometry
Endothelial Cells
Alleles
Maintenance
Gene Expression
Messenger RNA

Keywords

  • Endoderm
  • Hemogenic endothelium
  • Mesoderm
  • Sox17
  • Ventral pancreas

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Choi, E., Kraus, M. R. C., Lemaire, L. A., Yoshimoto, M., Vemula, S., Potter, L. A., ... Magnuson, M. A. (2012). Dual lineage-specific expression of Sox17 during mouse embryogenesis. Stem Cells, 30(10), 2297-2308. https://doi.org/10.1002/stem.1192

Dual lineage-specific expression of Sox17 during mouse embryogenesis. / Choi, Eunyoung; Kraus, Marine R C; Lemaire, Laurence A.; Yoshimoto, Momoko; Vemula, Sasidhar; Potter, Leah A.; Manduchi, Elisabetta; Stoeckert, Christian J.; Grapin-Botton, Anne; Magnuson, Mark A.

In: Stem Cells, Vol. 30, No. 10, 10.2012, p. 2297-2308.

Research output: Contribution to journalArticle

Choi, E, Kraus, MRC, Lemaire, LA, Yoshimoto, M, Vemula, S, Potter, LA, Manduchi, E, Stoeckert, CJ, Grapin-Botton, A & Magnuson, MA 2012, 'Dual lineage-specific expression of Sox17 during mouse embryogenesis', Stem Cells, vol. 30, no. 10, pp. 2297-2308. https://doi.org/10.1002/stem.1192
Choi E, Kraus MRC, Lemaire LA, Yoshimoto M, Vemula S, Potter LA et al. Dual lineage-specific expression of Sox17 during mouse embryogenesis. Stem Cells. 2012 Oct;30(10):2297-2308. https://doi.org/10.1002/stem.1192
Choi, Eunyoung ; Kraus, Marine R C ; Lemaire, Laurence A. ; Yoshimoto, Momoko ; Vemula, Sasidhar ; Potter, Leah A. ; Manduchi, Elisabetta ; Stoeckert, Christian J. ; Grapin-Botton, Anne ; Magnuson, Mark A. / Dual lineage-specific expression of Sox17 during mouse embryogenesis. In: Stem Cells. 2012 ; Vol. 30, No. 10. pp. 2297-2308.
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