Dural repair reduces connective tissue scar invasion and cystic cavity formation after acute spinal cord laceration injury in adult rats

Christopher Iannotti, Y. Ping Zhang, Lisa B E Shields, Yingchun Han, Darlene A. Burke, Xiao-Ming Xu, Christopher B. Shields

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

This study examined whether duraplasty after acute cervical laceration spinal cord injury (SCI) in a rat model could (1) improve cerebrospinal fluid (CSF) circulation adjacent to the injury; (2) minimize connective tissue scarring; and (3) reduce post-traumatic inflammation and cystic cavitation. Following a transverse dural/arachnoid incision and C5-6 dorsal spinal hemisection, a 5-mm2 cadaveric dura mater allograft was placed over the lesion and fixed with fibrin glue (n = 12). Control animals received an identical dural/arachnoid incision and cervical dorsal hemisection without dural repair (n = 12). At 1, 5, and 10 weeks post-injury, plain film myelograms were obtained to characterize CSF circulation, and stereological methods were used to compare the extent of tissue sparing between the two groups. Immunohistochemical studies were performed to assess the degree of inflammation (ED-1), connective tissue scarring (laminin and type IV collagen), and reactive astrogliosis (GFAP). Our results indicate that dural allograft can improve CSF flow adjacent to the site of injury, which may be due to reduced meningeal fibrosis/scarring at the lesion site. Stereological analysis demonstrated that duraplasty resulted in a significant reduction in lesion volume at each time-point (p <0.01) associated with a nearly complete attenuation of post-traumatic cystic cavitation (p <0.001). Immunofluorescence studies demonstrated that duraplasty reduced the infiltration of ED-1-positive macrophages/microglia into and surrounding the lesion site, which may be responsible for the marked reduction in secondary injury following duraplasty. We conclude that duraplasty following acute spinal cord laceration may (1) improve CSF flow by limiting meningeal fibrosis; (2) reduce connective tissue scar formation; and (3) attenuate macrophage accumulation and progressive secondary injury.

Original languageEnglish (US)
Pages (from-to)853-865
Number of pages13
JournalJournal of Neurotrauma
Volume23
Issue number6
DOIs
StatePublished - Jun 2006
Externally publishedYes

Fingerprint

Spinal Cord Injuries
Connective Tissue
Cicatrix
Cerebrospinal Fluid
Wounds and Injuries
Arachnoid
Allografts
Fibrosis
Macrophages
Inflammation
Dura Mater
Fibrin Tissue Adhesive
Collagen Type IV
Lacerations
Microglia
Laminin
Motion Pictures
Fluorescent Antibody Technique

Keywords

  • Cerebrospinal fluid
  • Cystic cavitation
  • Dorsal hemisection
  • Dura mater
  • Duraplasty
  • Fibrin glue
  • Inflammation
  • Laceration
  • Spinal cord injury
  • Vibraknife

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Dural repair reduces connective tissue scar invasion and cystic cavity formation after acute spinal cord laceration injury in adult rats. / Iannotti, Christopher; Zhang, Y. Ping; Shields, Lisa B E; Han, Yingchun; Burke, Darlene A.; Xu, Xiao-Ming; Shields, Christopher B.

In: Journal of Neurotrauma, Vol. 23, No. 6, 06.2006, p. 853-865.

Research output: Contribution to journalArticle

Iannotti, Christopher ; Zhang, Y. Ping ; Shields, Lisa B E ; Han, Yingchun ; Burke, Darlene A. ; Xu, Xiao-Ming ; Shields, Christopher B. / Dural repair reduces connective tissue scar invasion and cystic cavity formation after acute spinal cord laceration injury in adult rats. In: Journal of Neurotrauma. 2006 ; Vol. 23, No. 6. pp. 853-865.
@article{5fd9f6df59d24f48b438f744321e2e93,
title = "Dural repair reduces connective tissue scar invasion and cystic cavity formation after acute spinal cord laceration injury in adult rats",
abstract = "This study examined whether duraplasty after acute cervical laceration spinal cord injury (SCI) in a rat model could (1) improve cerebrospinal fluid (CSF) circulation adjacent to the injury; (2) minimize connective tissue scarring; and (3) reduce post-traumatic inflammation and cystic cavitation. Following a transverse dural/arachnoid incision and C5-6 dorsal spinal hemisection, a 5-mm2 cadaveric dura mater allograft was placed over the lesion and fixed with fibrin glue (n = 12). Control animals received an identical dural/arachnoid incision and cervical dorsal hemisection without dural repair (n = 12). At 1, 5, and 10 weeks post-injury, plain film myelograms were obtained to characterize CSF circulation, and stereological methods were used to compare the extent of tissue sparing between the two groups. Immunohistochemical studies were performed to assess the degree of inflammation (ED-1), connective tissue scarring (laminin and type IV collagen), and reactive astrogliosis (GFAP). Our results indicate that dural allograft can improve CSF flow adjacent to the site of injury, which may be due to reduced meningeal fibrosis/scarring at the lesion site. Stereological analysis demonstrated that duraplasty resulted in a significant reduction in lesion volume at each time-point (p <0.01) associated with a nearly complete attenuation of post-traumatic cystic cavitation (p <0.001). Immunofluorescence studies demonstrated that duraplasty reduced the infiltration of ED-1-positive macrophages/microglia into and surrounding the lesion site, which may be responsible for the marked reduction in secondary injury following duraplasty. We conclude that duraplasty following acute spinal cord laceration may (1) improve CSF flow by limiting meningeal fibrosis; (2) reduce connective tissue scar formation; and (3) attenuate macrophage accumulation and progressive secondary injury.",
keywords = "Cerebrospinal fluid, Cystic cavitation, Dorsal hemisection, Dura mater, Duraplasty, Fibrin glue, Inflammation, Laceration, Spinal cord injury, Vibraknife",
author = "Christopher Iannotti and Zhang, {Y. Ping} and Shields, {Lisa B E} and Yingchun Han and Burke, {Darlene A.} and Xiao-Ming Xu and Shields, {Christopher B.}",
year = "2006",
month = "6",
doi = "10.1089/neu.2006.23.853",
language = "English (US)",
volume = "23",
pages = "853--865",
journal = "Journal of Neurotrauma",
issn = "0897-7151",
publisher = "Mary Ann Liebert Inc.",
number = "6",

}

TY - JOUR

T1 - Dural repair reduces connective tissue scar invasion and cystic cavity formation after acute spinal cord laceration injury in adult rats

AU - Iannotti, Christopher

AU - Zhang, Y. Ping

AU - Shields, Lisa B E

AU - Han, Yingchun

AU - Burke, Darlene A.

AU - Xu, Xiao-Ming

AU - Shields, Christopher B.

PY - 2006/6

Y1 - 2006/6

N2 - This study examined whether duraplasty after acute cervical laceration spinal cord injury (SCI) in a rat model could (1) improve cerebrospinal fluid (CSF) circulation adjacent to the injury; (2) minimize connective tissue scarring; and (3) reduce post-traumatic inflammation and cystic cavitation. Following a transverse dural/arachnoid incision and C5-6 dorsal spinal hemisection, a 5-mm2 cadaveric dura mater allograft was placed over the lesion and fixed with fibrin glue (n = 12). Control animals received an identical dural/arachnoid incision and cervical dorsal hemisection without dural repair (n = 12). At 1, 5, and 10 weeks post-injury, plain film myelograms were obtained to characterize CSF circulation, and stereological methods were used to compare the extent of tissue sparing between the two groups. Immunohistochemical studies were performed to assess the degree of inflammation (ED-1), connective tissue scarring (laminin and type IV collagen), and reactive astrogliosis (GFAP). Our results indicate that dural allograft can improve CSF flow adjacent to the site of injury, which may be due to reduced meningeal fibrosis/scarring at the lesion site. Stereological analysis demonstrated that duraplasty resulted in a significant reduction in lesion volume at each time-point (p <0.01) associated with a nearly complete attenuation of post-traumatic cystic cavitation (p <0.001). Immunofluorescence studies demonstrated that duraplasty reduced the infiltration of ED-1-positive macrophages/microglia into and surrounding the lesion site, which may be responsible for the marked reduction in secondary injury following duraplasty. We conclude that duraplasty following acute spinal cord laceration may (1) improve CSF flow by limiting meningeal fibrosis; (2) reduce connective tissue scar formation; and (3) attenuate macrophage accumulation and progressive secondary injury.

AB - This study examined whether duraplasty after acute cervical laceration spinal cord injury (SCI) in a rat model could (1) improve cerebrospinal fluid (CSF) circulation adjacent to the injury; (2) minimize connective tissue scarring; and (3) reduce post-traumatic inflammation and cystic cavitation. Following a transverse dural/arachnoid incision and C5-6 dorsal spinal hemisection, a 5-mm2 cadaveric dura mater allograft was placed over the lesion and fixed with fibrin glue (n = 12). Control animals received an identical dural/arachnoid incision and cervical dorsal hemisection without dural repair (n = 12). At 1, 5, and 10 weeks post-injury, plain film myelograms were obtained to characterize CSF circulation, and stereological methods were used to compare the extent of tissue sparing between the two groups. Immunohistochemical studies were performed to assess the degree of inflammation (ED-1), connective tissue scarring (laminin and type IV collagen), and reactive astrogliosis (GFAP). Our results indicate that dural allograft can improve CSF flow adjacent to the site of injury, which may be due to reduced meningeal fibrosis/scarring at the lesion site. Stereological analysis demonstrated that duraplasty resulted in a significant reduction in lesion volume at each time-point (p <0.01) associated with a nearly complete attenuation of post-traumatic cystic cavitation (p <0.001). Immunofluorescence studies demonstrated that duraplasty reduced the infiltration of ED-1-positive macrophages/microglia into and surrounding the lesion site, which may be responsible for the marked reduction in secondary injury following duraplasty. We conclude that duraplasty following acute spinal cord laceration may (1) improve CSF flow by limiting meningeal fibrosis; (2) reduce connective tissue scar formation; and (3) attenuate macrophage accumulation and progressive secondary injury.

KW - Cerebrospinal fluid

KW - Cystic cavitation

KW - Dorsal hemisection

KW - Dura mater

KW - Duraplasty

KW - Fibrin glue

KW - Inflammation

KW - Laceration

KW - Spinal cord injury

KW - Vibraknife

UR - http://www.scopus.com/inward/record.url?scp=33745500441&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745500441&partnerID=8YFLogxK

U2 - 10.1089/neu.2006.23.853

DO - 10.1089/neu.2006.23.853

M3 - Article

C2 - 16774471

AN - SCOPUS:33745500441

VL - 23

SP - 853

EP - 865

JO - Journal of Neurotrauma

JF - Journal of Neurotrauma

SN - 0897-7151

IS - 6

ER -